DNA encoding the 15kD outer membrane protein of Haemophilus infl

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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536 221, 536 231, 536 237, 530300, 4241841, 4241851, 4242561, 435 6, A61K 3902, A61K 39102, C07H 2102, C12P 2106

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055039928

ABSTRACT:
Murine monoclonal antibodies directed against a novel outer membrane protein (OMP) of Haemophilus influenzae have been isolated and characterized. The gene encoding of the outer membrane protein has also been isolated and characterized. Portions of the DNA sequence of the 15 kD OMP gene are useful as probes to diagnose the presence of Haemophilus influenzae in samples. These DNA's also make available polypeptide sequences of immunoreactive epitopes encoded within the gene, thus permitting the production of polypeptides which are useful as standards or reagents in diagnostic tests and/or as components of vaccines. Monoclonal antibodies directed against epitopes of the 15 kD OMP are also useful for diagnostic tests and as therapeutic agents for passive immunization.

REFERENCES:
Young et al PNAS 80:1194-1198, 1983.
Dugourd et al, Abstracts of Gen. Meeting ASM p. 88, Abstract B-373.
Green et al Infection and Immunity 55: 2878-2883, 1987.
Grass et al, Abstracts of Gen Meeting of ASM p. 105, Abst. D-57.
Humel et al J. Med Microbiol 23: 163-70, 1987, Abstract Only.

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