DNA encoding single chain monellin

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4352523, 4353201, 435325, 435419, 536 236, C12P 2106, C12N 506, C12N 1503, C07H 2104

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056703392

ABSTRACT:
Single-chain analogs of the naturally occurring two-chain peptide monellin retain the sweetening properties of the natural protein and are stable under conditions which would otherwise destabilize the native peptide. A covalent linkage joins peptides corresponding to portions of the A and B chains of the naturally occurring protein.

REFERENCES:
patent: 3998798 (1976-12-01), Cagan et al.
patent: 5487983 (1996-01-01), Kim et al.
De Vos et al., Three-dimensional structure of thaumatin I, an intensely sweet protein; Proc. Natl. Acad. Sci. USA (1985) 82:1406-1409.
Ogata et al., Crystal structure of the intensely sweet protein monellin; Nature (1987) 328:739-742.
Morris et al., Characterization of monellin, a protein that tastes sweet; J. Biol. Chem. (1973) 248:534-539.
Cagan R.H., Chemostimulatory protein: A new type of taste stimulus; Science (1973) 181:32-25.
Bohak et al., The structure of monellin and its relation to the sweetness of the protein; Biochim. Biophys. Acta. (1976) 427:153-170.
Hudson et al., Mass spectrometric sequencing of proteins. The structure of subunit I of monellin; Biochem. Biophys. Res. Comm. (1976) 71:212-220.
Van der Wel et al., Characterization of the sweet-tasting protein from dioscoreophyllum cumminsi (Stapf) diels; FEBS Lett (1973) 29:181-183.
Frank et al., The complete amino acid sequences of both subunits of the sweet protein monellin; Z. Physiol. Chem. (1976) 357:585-592.
Morris et al., Purification of monellin, the sweet principle of dioscoreophyllum cumminsii; Biochim. Biophys. Acta. (1972 261:114-122.
Protein Stability & Stabilization Through Protein Engineering, Nosoh et al., 1991, Ellis Horwood, Pub; N.Y. p. 62.

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