DNA encoding polypeptide having nitrile hydratase activity and m

Details DNA encoding polypeptide having nitrile hydratase activity and m DNA encoding polypeptide having nitrile hydratase activity and m

1989-06-28

1992-07-14

Ellis, Joan

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435129, 4352351, 4353201, 4352523, 43525233, 435227, 435 91, 4351723, 536 27, 530350, 935 19, 935 29, 935 41, 935 56, 935 61, 935 73, 935 72, 935 82, C12P 2100, C12P 1934, C12P 1302, C12N 1500

Patent

active

051302357

ABSTRACT:
A DNA which encodes polypeptide carrying two specific amino acid sequences and having nitrile hydratase activity; a method for producing nitrile hydratase by incubating a transformant made by the transformation with a recombinant DNA prepared by integrating the foregoing DNA into a vector in a medium and harvesting nitrile hydratase accumulated in the medium; and a method for producing amides by incubating the foregoing transformant and then converting nitriles into corresponding amides by the action of the obtained nitrile hydratase or by making a culture solution, isolates, treated cells or their immobilized products act upon nitriles to produce corresponding amides.

REFERENCES:
Ikehata et al., Eur. J. Biochem vol. 181 pp. 563-570 (1989).

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