DNA encoding peptide hormone that inhibits digestion in insects

Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Plant cell or cell line – per se – is pest or herbicide...

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435419, 4353201, 4352351, 4352523, 4352542, 530329, A61K 3804, C12N 1511

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active

056291965

ABSTRACT:
The subject invention concerns novel peptides which have the property of interfering with the biosynthesis of the enzyme trypsin. This property enables the use of these peptides to, for example, inhibit the formation of progeny in blood-ingesting insects, since trypsin is an essential enzyme for food digestion which provides the essential building blocks for egg development in such insects.

REFERENCES:
patent: 5011909 (1991-04-01), Borovsky et al.
patent: 5130253 (1992-07-01), Borovsky et al.
Borovsky, Dov (1985) "Isolation and Characterization of Highly Purified Mosquito Oostatic Hormone" Archives of Insect Biochemistry and Physiology 2:333-349.
Borovsky, Dov (1988) "Oostatic Hormone Inhibits Biosynthesis of Midgut Proteplytic Enzymes and Egg Development in Mosquitoes" Archives of Insect Biochemistry and Physiology 7:187-210.
Borovsky, D. et al. (1990) "Mosquito oostatic factor: a novel decapeptide modulating trypsin-like enzyme biosynthesis in the midgut" FASEB J. 4:3015-3020.
Rayne, R.C., M. O'Shea (1992) "Inactivation of Neuropeptide Hormones (AKH I and AKH II) Studied In Vivo and In Vitro" Insect Biochem. Molec. Biol. 22(1):25-34.
Schwartz, J.-C. et al. (1981) "Biological Inactivation of Enkephalins and the Role of Enkephalin-Dipeptidyl-Carboxypeptidase (Enkephalinase) as Neuropeptidase" Life Sciences 29:1715-1740.
Charbonneau, Harry (1989) "Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (>5 nmol) of Pure or Partially Purified Protein" A Practical Guide to Protein and Peptide Purification for Microsequencing pp. 15-30.
Sober, H.A. (1968) "Handbook of Biochemistry" The Chemical Rubber Co., Cleveland, Ohio, p. C70.
Borovsky, D. et al. (1992) "Development of Specific RIA and ELISA to Study Trypsin Modulating Oostatic Factor in Mosquitoes" Archives of Insect Biochemistry and Physiology 21:13-21.

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