DNA encoding novel tissue plasminogen activator derivatives havi

Details DNA encoding novel tissue plasminogen activator derivatives havi DNA encoding novel tissue plasminogen activator derivatives havi

1990-11-19

1993-09-14

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435226, 4352402, 4353201, 536 232, 536 234, 536 235, C12N 964, C12N 948, C12N 500, C12N 1500

Patent

active

052448061

ABSTRACT:
The invention discloses modified forms of the enzyme tissue plasminogen activator (t-PA) in which recombinant DNA techniques are utilized to modify the structure of the t-PA cDNA to express a modified t-PA which retains the ability to activate plasminogen yet binds less efficiently to plasmin inhibitor. The invention provides novel DNA compounds and recombinant DNA expression vectors that encode modified human t-PA wherein all or a portion of the kringle protein domains of native human t-PA are removed. Both eukaryotic and prokaryotic expression vectors containing the modified t-PA DNA have been constructed and used to transform Chinese hamster ovary cells and Escherichia coli cells.

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