Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2004-08-05
2008-08-19
Pak, Michael (Department: 1646)
Chemistry: molecular biology and microbiology
Vector, per se
C435S325000, C536S023500
Reexamination Certificate
active
07413895
ABSTRACT:
A novel amino acid transporter molecule mediating transportation of amino acids, which are nutrients essentially required in the survival and proliferation of various normal cells constituting a living body and various pathology-associated abnormal cells such as tumor cells, into cells and being expressed specifically in tumor cells compared with normal cells; and drugs for treating various pathogenic conditions such as tumor (cancer) which are obtained by identifying and isolating the above amino acid transporter molecule and identifying a substance capable of inhibiting the biological activity and/or expression of this molecule. Intensive studies were made to identify a tumor cell membrane surface molecule associating or interacting with a cell membrane surface 4F2hc molecule seemingly playing an important role in the activation of an unknown amino acid transporter. As a result, a gene encoding the novel amino acid transporter molecule, which mediates the incorporation of various neutral amino acids, various drugs or physiological substances into cells, has been found out and a substance capable of inhibiting the incorporation of amino acids via this molecule and thus inhibiting the proliferation of tumor cells has been also found out.
REFERENCES:
patent: 5843727 (1998-12-01), Hillman et al.
Prasad et al., “Human LAT1, a Subunit of System L Amino Acid Transporter: Molecular Cloning and Transport Function,”Biochem. Biophys. Res. Commun. 255:2 pp. 283-288 (1999).
Tsurudome et al., “Cutting Edge: Primary Structure of the Light Chain of Fusion Regulatory Protein-1/CD98/4F2 Predicts a Protein with Multiple Transmembrane Domains That is Almost Identical to the Amino Acid Transporter E16,”J. Immunol. 162:5 pp. 2462-2466 (1999).
Mastroberardino et al., “Amino-acid transport by heterodimers of 4F2hc/CD98 and members of a permease family,”Nature395:6699 pp. 288-291 (1999).
Kanai et al., “Expression Cloning and Characterization of a Transporter for Large Neutral Amino Acids Activated by the Heavy Chain of 4F2 Antigen,” (CD98),J. Biol. Chem. 273:37 pp. 23629-23632 (1998).
Nakamura et al., “4F2(CD98) Heavy Chain is Associated Covalently with an Amino Acid Transporter and Controls Intracellular Trafficking and Membrane Topology of 4F2 Heterodimer,”Journal of Biol. Chem. 274:5 p. 3009-3016 (1999).
Haynes et al., “Characterization of a Monoclonal Antibody (4F2) that Binds to Human Monocytes and a Subset of Activated Lymphocytes,”J. Immunol. 128:4 1409-1414 (1981).
Hemler et al., “Characterization of the Antigen Recognized by the Monoclonal Antibody (4F2): Different Molecular Forms on Human T and B Lymphoblastoid Cell Lines,”J. Immunol. 129:2 pp. 623-628 (1982).
Teixeira et al., “Primary Structure of Human 4F2 Antigen Heavy Chain Predicts a Transmembrane Protein with a Cytoplasmic NH2 Terminus,”J. Biol. Chem. 262:20 pp. 9574-9580 (1987).
Lumadue et al., “Cloning, sequence analysis, and expression of thelarge subunit of the human lymphocyte activation antigen 4F2,”Proc. Natl. Acad. Sci. USA84:24 pp. 9204-9208 (1987).
Quackenbush et al., “Molecular Cloning of complementary DNAs Encoding the Heavy Chain of the Human 4F2 Cell Surface Antigen: A Type II Membrane Glycoprotein Involved in Normal and Neoplastic Cell Growth,”Proc. Natl. Acad. Sci. USA84:18 pp. 6526-6530 (1987).
Broer et al., “The 4F2hc surface antigen is necessary for expression of system L-like neutral amino acid-transport activity in C6-BU-1 rat glioma cells: evidence from expression studies inXenopus laevisoocytes,”Biochem. J. 312:3 pp. 863-870 (1995).
Yao et al., “Cloning and Functional Expression of a cDNA from Rat Jejunal Epithemlium Encoding a Protein (4F2hc) with System y+L Amino Acid Transport Activity,”Biochem. J. 330:2 pp. 745-752 (1998).
Gaugitsch et al., “A Novel Expressed, Integral Membrane Protein Linked to Cell Activation,”J. Biol. Chem. 267:16 pp. 11267-11273 (1992).
Torrents et al., “Identification and Characterization of a Membrane Protein (y+L Amino Acid Transporter-1) That Associates with 4F2hc to Encode the Amino Acid Transport Activity y+L,”The Journal of Biological Chemistry273:49 pp. 32437-32445 (1998).
Estevez et al., “The amino acid transport system y+L/4F2hc is a heteromultimeric complex,”The FASEB Journal12:1319-1329 (1998).
Broer et al., “Discrimination of two amino acid transport activities in 4F2 heavy chain-expressingXenopus laevisoocytes,”Biochem. J. 333:549-554 (1998).
Wells et al., “The 4F2 Antigen Heavy Chain Induces Uptake of Neutral and Dibasic Amino Acids inXenopusOocytes,”The Journal of Biological Chemistry267:2 pp. 15285-15288 (1992).
Gottesdiener et al., “Isolation and Structural Characterization of the Human 4F2 Heavy-Chain Gene, and Inducible Gene Involved in T-Lymphocyte Activation,”Molecular and Cellular Biology8:9 pp. 3809-3819 (1988).
Spindler et al., “Characterization of early aldosterone-induced RNAs identified in A6 kidney epithelia,”Pflugers Arch. 434:323-331(1997).
Broer et al., “Association of 4F2hc with light chains LAT1, LAT2 or y+LAT2 requires different domains,”Biochem. J. 355:725-731 (2001).
D K. Kim et al., “Expression of L-type Amino Acid Transporter 1 (LAT1) and 4F2 Heavy Chain (4F2hc) in Oral Squamous Cell Carcinoma and its Precusor Lesions”, Anti-Cancer Research, vol. 24, pp. 1671-1676 (2004).
J.H. Yoon et al., “Expression and Functional Characterization of the System L Amino Acid Transporter in KB Human Oral Epidermoid Carcinoma Cells”, Cancer Letters, vol. 205, pp. 215-226 (2004).
O. Yanagida et al., “human L-type Amino Acid Transporter 1 (LAT1): Characterization of Function and Expression in Tumor Cell Lines”, Bioch. et Bioph. Acta, vol. 1514, pp. 291-302 (2001).
J.H. Yoon et al., “Amino Acid Transport System L is Differently Expressed in Human Normal Oral Keratinocytes and Human Oral Cancer Cells”, Cancer Letters, vol. 222, pp. 237-245 (2005).
S. Tamai et al., “Expression of L-Type Amino Acid Transporter 1 in a Rat Model of Liver Metastasis: Positive Correlation with Tumor Size”, Cancer Detection and Prevention, vol. 25, No. 5, pp. 439-445 (2001).
D.K. Kim et al., “Characterization of the System L Amino Acid Transporter in T24 Human Bladder Carcinoma Cells”, Biosh. et Bioph. Acta, vol. 1565, pp. 112-122 (2002).
K. Nakanishi et al., “LAT1 Expression in Normal Lung and in Atypical Adenomatous Hyperplasia and Adenocarcinoma of the Lung”, Virchows Arch, vol. 448, pp. 142-150 (2006).
D.K. Kim et al., “System L-Amino Acid Transporters are Differently Expressed in Rat Astrocyte and C6 Glioma Cells”, Neuroscience Research, vol. 50, pp. 437-446 (2004).
H. Nawashiro et al., “L-Type Amino Acid Transporter 1 as a Potential Molecular Target in Human Astrocytic Tumors”, Int. J. Cancer, vol. 119, pp. 484-492 (2006).
Endou Hitoshi
Kanai Yoshikatsu
Corless Peter F.
Edwards Angell Palmer & & Dodge LLP
Japan Science and Technology Corporation
O'Day Christine C.
Pak Michael
LandOfFree
DNA encoding neutral amino acid transporter does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with DNA encoding neutral amino acid transporter, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA encoding neutral amino acid transporter will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4007190