DNA encoding mammalian phosphodiesterases

Details DNA encoding mammalian phosphodiesterases DNA encoding mammalian phosphodiesterases

1994-08-29

1997-02-11

Jacobson, Dian C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435199, 4352523, 4352542, 4353201, 435325, 536 232, C12N 116, C12N 916, C12N 1555, C12N 1563

Patent

active

056020198

ABSTRACT:
The present invention relates to novel purified and isolated nucleotide sequences encoding mammalian Ca.sup.2+ /calmodulin stimulated phosphodiesterases (CaM-PDEs) and cyclic-GMP-stimulated phosphodiesterases (cGS-PDEs). Also provided are the corresponding recombinant expression products of said nucleotide sequences, immunological reagents specifically reactive therewith, and procedures for identifying compounds which modulate the enzymatic activity of such expression products.

REFERENCES:
Ausubel et al., eds., Current Protocols in Molecular Biology, 1:1.7.1-1.7.2 and 9.2.1-9.2.3, John Wiley & Sons, New York (1989).
Beavo, "Multiple Isozymes of Cyclic Nucleotide Phosphodiesterase", Advances in Second Messenger and Phosphoprotein Research, 22: 1-38 (1988).
Beavo, "Multiple Phosphodiesterase Isoenzymes Background, Nomenclature and Implications", pp. 3-15; Cyclic Nucleotide Phosphodiesterases: Structure, Regulation and Drug Action, J. Beavo and Houslay, M.D., Eds.; John Wiley & Sons, Ltd., New York (1990).
Birnstiel et al., "Transcription Termination and 3' Processing: The End Is in Sight!", Cell, 41:349-359 (1985).
Bourne et al., "Somatic Genetic Analysis of Cyclic AMP Action: Characterization of Unresponsive Mutants", J. Cell. Physiol., 85:611-620 (1985).
Bradford, "A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding", Analytical Biochem., 72:248-254 (1976).
Chen et al., "Molecular Analysis of cDNA Clones and the Corresponding Genomic Coding Sequences of the Drosophila dunce.sup.+ Gene, the Structural Gene for cAMP Phosphodiesterase", Proc. Nat'l. Acad. Sci. (USA), 83:9313-9317 (1986).
Chomczynski et al., "Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction", Analytical Biochem., 162:156-159 (1987).
Colicelli et., "Isolation and Characterization of a Mammalian Gene Encoding a High-Affinity cAMP Phosphodiesterase", Proc. Nat'l. Acad. Sci. (USA), 86:3599-3603 (1989).
Davis, "Molecular Genetics of the Cyclic Nucleotide Phosphodiesterases", pp. 227-241 in Cyclic Nucleotide Phosphodiesterases: Structure, Regulation and Drug Action, J. Beavo and Houslay, M.D., Eds.: John Wiley & Sons, Ltd., New York (1990).
Davis et al., "Cloning and Characterization of Mammalian Homologs of the Drosophila dunce.sup.+ Gene", Proc. Nat'l Acad. Sci. (USA), 86:3604-3608 (1989).
Devereux et al., "A Comprehensive Set of Sequence Analysis Programs for the VAX", Nucleic Acids Res., 12:387-395 (1984).
Erneux et al., "A Mechanism in the Control of Intracellular cAMP Levels: The Activation of a Calmodulin-Sensitive Phosphodiesterase by a Rise of Intracellular Free Calcium", Mol. Cell. Endocrinol., 43:123-134 (1985).
Faure et al., "Disruption of Dictyostelium discoideum Morphogenesis by Overproduction of cAMP Phosphodiesterase", Proc. Nat'l. Acad. Sci. (USA), 85:8076-8080 (1988).
Feinberg et al., "A Technique for Radiolabeling DNA Restriction Endonuclease Fragments to High Specific Activity", Analytical Biochem., 137:266-267 (1984).
Greenberg et al., "Enzymatic Regulation of the Concentration of Cyclic GMP in Mouse Brain", Neuropharmacology, 17:737-745 (1978).
Hansen et al., "Differential Recognition of Calmodulin-Enzyme Complexes by a Conformation-Specific Anti-Calmodulin Monoclonal Antibody", J. Biol. Chem., 261:14636-14645 (1986).
Hansen et al, "Purification of Calmodulin-Stimulated Cyclic Nucleotide Phosphodiesterase by Using Conformation-Specific Monoclonal Antibody Chromatography", Proc. Nat'l. Acad. Sci. (USA), 79:2788-2792 (1982).
Hansen et al., "Purification of Two Calcium/Calmodulin-Stimulated Cyclic Nucleotide Phosphodiesterase by Monoclonal Antibody Affinity Chromatography", Meth. Enzymol., 159:543-557 (1988).
Hashimoto et al., "Regulation of Ca.sup.2+ /Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase by the Autophosphorylated Form of Ca.sup.2+ /Calmodulin-Dependent Protein Kinase II", J. Biol. Chem., 264:10884-10887 (1989).
Henikoff, "Unidirectional Digestion with Exonuclease III Creates Targeted Breakpoints for DNA Sequencing", Gene, 28:351-359 (1984).
Kincaid et al., "Differential Localization of Calmodulin-Dependent Enzymes in Rat Brain: Evidence for Selective Expression of Cyclic Nucleotide Phosphodiesterase in Specific Neurons", Proc. Nat'l. Acad. Sci. (USA), 84:1118-1122 (1987).
Kozak, "The Scanning Model for Translation: An Update", J. Cell. Biol., 108:229-241 (1989).
Krinks et al., "Reversible and Irreversible Activation of Cyclic Nucleotide Phosphodiesterase: Separation of the Regulatory and Catalytic Domains by Limited Proteolysis", Advances in Cyclic Nuleotide and Protein Phosphorylation Research, 16:31-47 (1984).
LaPorte et al., "Cross-Linking of Iodine-125-Labeled, Calcium-Dependent Regulatory Protein to the Ca.sup.2+ -Sensitive Phosphodiesterase Purified from Bovine Heart", Biochemistry, 18:2820-2825 (1979).
LeTrong et al., "Amino Acid Sequence of the Cyclic GMP Stimulated Cyclic Nucleotide Phosphodiesterase from Bovine Heart", Biochemistry, 29:10280-10288 (1990).
Livi et al., "Cloning and Expression of cDNA for a Human Low-K.sub.M Rolipram-Sensitive Cyclic AMP Phosphodiesterase", Mol. Cell. Biol., 10;2678-2686 (1990).
Manganiello et al., "Cyclic GMP-Stimulated Cyclic Nucleotide Phosphodiesterases", pp. 62-85 in Cyclic Nucleotide Phosphodiesterases: Structure, Regulation and Drug Action, Beavo, J. and Houslay, M. D., Eds.: John Wiley & Sons, Ltd., New York (1990).
Maniatis et al., Molecular Cloning: A Laboratory Manual, pp. 326-328, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1982).
Martins et al., "Purification and Characterization of a Cyclic GMP-Stimulated Cyclic Nucleotide Phosphodiesterase from Bovine Tissues", J. Biol. Chem., 257:1973-1979 (1982).
Nikawa et al., "Cloning and Characterization of the Low-Affinity Cyclic AMP Phosphodiesterase Gene of Saccaromyces cerevisiae", Mol. Cell. Biol., 7:3629-3636 (1987).
Nomenclature Committee of the International Union of Biochemistry (NCIUB), "Nomenclature for Incompletely Specified Bases in Nucleic Acid Sequences", J. Biol. Chem., 261:13-17 (1986).
Novack et al., "Sequence Comparison of the 63-, 61-and 59-kDa Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterases", Biochemistry, 30:7940-7947 (1991).
Ovchinnikov et al., "Cyclic GMP Phosphodiesterase from Bovine Retina", FEBS, 223:169-173 (1987).
Sanger et al., "DNA Sequencing with Chain-Terminating Inhibitors", Proc. Nat'l. Acad. Sci. (USA), 74:5463-5467 (1977).
Sass et al., "Cloning and Characterization of the High-Affinity cAMP Phosphodiesterase of Saccromyces cerevisiae", Proc. Nat'l. Acad. Sci. (USA), 83:9303-9307 (1986).
Seed, "An LFA-3 cDNA encodes a Phospholipid-Linked Membrane Protein Homologous to Its Receptor CD2", Nature, 329:840-843 (1987).
Sharma et al., "Demonstration of Bovine Brain Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase Isozymes by Monoclonal Antibodies", J. Biol. Chem., 259:9248-9254 (1984).
Sharma et al., "Differential Regulation of Bovine Brain Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase Isozymes by Cyclic AMP-Dependent Protein Kinase and Calmodulin-Dependent Phosphatase", Proc. Nat'l. Acad. Sci. (USA), 82:2603-2607 (1985).
Sharma et al., "Purification and Characterization of Bovine Lung Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase", J. Biol. Chem., 261:14160-14166 (1986).
Sherman et al., Methods in Yeast Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1986).
Short et al., "ZAP: A Bacteriophage .gamma. Expression Vector with in vivo Excision Properties", Nucleic Acids Res., 16:7583-7600 (1988).
Sonnenburg et al., "Molecular Cloning of a Cyclic GMP-Stimulated Cyclic Nucleotide Phosphodiesterase cDNA", J. Biol. Chem., 266(26):17655-17661 (1991).
Stroop et al., "Direct Photolabeling of the cGMP-Stimulated Cyclic Nucleotide Phosphodiesterase", J. Biol. Chem., 264:13718-13725 (1989).
Swinnen et al., "Molecular Cloning of Rat Homologues of the Drosophila melanogaster dunce cAMP Phosphodiesterase: Evidence for a Family of Genes", Proc. Nat'l. Acad. Sci. (USA),

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