DNA encoding hyaluronate synthase

Details DNA encoding hyaluronate synthase DNA encoding hyaluronate synthase

1989-08-29

1991-05-14

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435193, 4352523, 43525233, 4353201, 435849, 435885, 536 26, 536 27, 536 28, 935 22, 935 27, 935 55, 935 56, 935 60, 935 72, 935 73, C12P 1904, C12N 120, C12N 910, C07H 1512

Patent

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050155776

ABSTRACT:
Disclosed are DNA segments encoding hyaluronic acid synthase which are employed to construct recombinant cells useful in the production of hyaluronate synthase or hyaluronic acid (HA). In preferred aspects, chromosomal DNA from Streptococcus equisimilis is partially digested with EcoRI and the resultant fragments are ligated to form recombinant vectors. These vectors are useful in the transformation of host cells such as E. coli or Streptococcal hosts. Resultant transformants are screened by novel screening assays to identify colonies which have incorporated HA synthase DNA in a form that is being actively transcribed into the corresponding HA synthase enzyme. These colonies may be selected and employed in the production of the enzyme itself or its product, HA.

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