DNA encoding humanized antibodies specific for the ganglioside G

Details DNA encoding humanized antibodies specific for the ganglioside G DNA encoding humanized antibodies specific for the ganglioside G

1995-05-10

1999-02-23

Caputa, Anthony C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 7021, 4351722, 4353201, 435328, 435330, 435332, 4353441, 435346, 5303873, 5303875, 5303877, 5303881, 5303882, 530867, 536 2353, 935 15, C12P 2104, C12P 2108, C12N 1500, C07H 2104

Patent

active

058742557

ABSTRACT:
Chimeric human antibody expression vectors are constructed by inserting the antibody heavy chain variable region-encoding cDNA and antibody light chain variable region-encoding cDNA isolated from hybridomas producing a mouse or rat monoclonal antibody reacting with the ganglioside GM.sub.2 respectively into an expression vector for use in animal cells which contains the human antibody heavy chain constant region- or human antibody light chain constant region-encoding cDNA. The expression vectors are introduced into animal cells and the transformant thus obtained is cultured for the production of a chimeric human antibody reacting with the ganglioside GM.sub.2.
In contrast to mouse monoclonal antibodies, the chimeric human antibodies of the invention will not cause anti-mouse immunoglobulin antibody production in the patient's body but shows a prolonged blood half-life, with a reduced frequency of adverse effects, so that it can be expected to be superior to mouse monoclonal antibodies in the efficacy in the treatment of human cancer, for instance.

REFERENCES:
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Morrison et al, PNAS vol. 81: 6851-6855, Nov. 1984.
Hodgson, Bio/Technology vol. 8:1245-1247, Dec. 8, 1990.
Morrison et al. Advances in Immunology 44, Dixon et al. Eds. Academic Press 198 pp. 65-92. Schlom et al. "Molecular Foundations of Oncology", Broder Ed., Williams & Wilkins, 1991 pp. 95-134.
Hakomori et al. in Monoclonal Antibodies and Functional Cell Lines Kennett et al., eds. 1985 pp. 67-100.
Kannagi et al., Handbook of Expt'l Immunology 4:1986, 117.1-117.21 Irie et al. Lancet, Apr. 8, 1989 786-7.

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