DNA encoding human granulocyte colony stimulating factor plasmid

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 691, 4351723, 4352523, 4353201, 536 231, 536 235, 536 241, C07K 15535, C12N 1527, C12N 1564, C12N 1566

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056817207

ABSTRACT:
Novel hG-CSF polypeptide derivatives having an amino acid sequence derived from the amino acid sequence of the human granulocyte colony stimulating factor polypeptide by substitution of at least one amino acid by a different amino acid and/or deletion of at least one amino acid, recombinant plasmids containing a DNA fragment insert coding for any of these hG-CSF polypeptide derivatives, microorganisms carrying one of such plasmids, methods of producing the hG-CSF polypeptide derivatives using the microorganisms, a monoclonal antibody binding to the hG-CSF polypeptide derivative, and chemically modified hG-CSF or derivatives thereof are disclosed.

REFERENCES:
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patent: 4904584 (1990-02-01), Shaw
Ayala et al. 1980. Modern Genetics. Benjamin/Cummings Publishing Co., Inc., Menlo Park, CA. pp. 45-48.
Bowie et al. 1990. Science 247: 1306-1310.
Cantrell et al. 1985. PNAS USA 82: 6250-6254.
Wills et al, Gene, 34, 1985, pp. 315-323
Zoller et al, Nuclear Acad Res 10 (20) 1982, pp. 6487-6100.

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