Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-12-07
2000-07-04
Elliott, George C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 691, 4352402, 4353201, 530 231, C12Q 168, C07H 2104
Patent
active
060837055
ABSTRACT:
This invention provides an isolated nucleic acid, vectors, transformed mammalian cells and non-human transgenic animals that encode and express normal or mutant .alpha. 1a, .alpha. 1b and .alpha. 1c adrenergic receptor genes. This invention also provides a protein, and an antibody directed to the protein and pharmaceutical compounds related to .alpha. 1a, .alpha. 1b and .alpha. 1c adrenergic receptors. This invention provides nucleic acid probes, and antisense oligonucleotides complementary to .alpha. 1a, .alpha. 1b and .alpha. 1c adrenergic receptor genes. This invention further provides methods for determining ligand binding, detecting expression, drug screening, and treatments for alleviating abnormalities associated with human .alpha. 1a, .alpha. 1b and .alpha. 1c adrenergic receptors.
REFERENCES:
patent: 4616017 (1986-10-01), Baldwin et al.
patent: 4661491 (1987-04-01), Regnier
patent: 4873191 (1989-10-01), Wagner et al.
patent: 5155218 (1992-10-01), Weinshank et al.
patent: 5556753 (1996-09-01), Bard et al.
patent: 5714381 (1998-02-01), Bard et al.
Cubbedu, L.X., American Heart Journal (1988) 116:133-162 (Exhibit 4).
Fraser, et al., "Autobodies and Monoclonal Antibodies in the Purification and Molecular Characterization of Neurotransmitter Receptors", J. Cell. Biochem. (1983) 21(3): 219-231 (Exhibit 5).
Ramarao, C.S., et al., "Genomic Organization and Expression of the Human .alpha..sub.1B -Adrenergic Receptor" J. Biol. Chem. (1992) 267(30): 21936-21945 (Exhibit 6).
Adham, N., et al., "Cloning of another human serotonin receptor (5-HT.sub.1F): A fifth 5HT.sub.1 receptor subtype coupled to the inhibition of Adenylate cyclase", Proc. Natl. Acad. Sci. USA (1993) 90: 408-412.
Bruno, J.F., et al., Molecular cloning and sequencing of a cDNA encoding a human .alpha..sub.1A -adrenergic receptor. Biochem. Biophys. Res. Com. 1991. 179(3): 1485-1490.
Cotecchia, et al., "Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor", Proc. Natl. Acad. Sci. (1988) 85: 7159-7163.
Cotten, M. and Birnstiel, M.L., Ribozyme mediated destruction of RNA in vivo, EMBO J. (1989) 8(12): 3861-3866.
DiBona, G.F., Hypertension and renal alpha adrenergic receptors, FASEB J. (1989) 3: 1993-1994.
Forray, C., et al., "The .alpha..sub.1 -Adrenergic Receptor that Mediates Smooth Muscle Contraction in Human Prostate Has the Pharmacological Properties of the Cloned Human .alpha..sub.1C Subtype" Molecular Pharmacol. (1994) 45: 703-708.
Gao, et al., "Isolation and characterization of the gene encoding the rat .alpha..sub.1B adrenergic receptor" Gene (1993) 131: 243-247.
Glover, D.M., Gene Cloning, Chapman & Hall, London 1984, p. 1-21.
Jones, C.R., et al., "Autoradiography of adrenoceptors in rat and human brain: .alpha.-adrenoceptor and idazoxan binding sites" Progress In Brain Research (1991) 88: 271-291.
Karson, E.M., et al., Prospects for human gene therapy, J. Reprod. Med. (1992) 37(6): 508-514.
Laz, T.M., et al., "The Rat Homologue of the Bovine .alpha..sub.1C -Adrenergic Receptor Shows the Pharmacological Properties of the classical .alpha..sub.1A Subtype" Molecular Pharmacol. (1994) 46: 414-422.
Lerner, R.A., Tapping the immunological repertoire to produce antibodies of predetermined specificity, Nature (1982) 299: 592-596.
Link, R., et al., Cloning of Two Mouse Genes Encoding .alpha..sub.2 -Adrenergic Receptor Subtypes and Identification of a Single Amino Acid in the Mouse .alpha..sub.2 -C10 Homolog Responsible for an Interspecies Variation in Antagonist Binding, Molecular Pharmacology (1992) 42: 16-27.
Lomasney, J.W., et al., Molecular cloning and expression of the cDNA for the .alpha..sub.1A -adrenergic receptor. J. Biol. Chem. 1991 266(10): 6365-6369.
Perala, M., et al., "Differential expression of two .alpha..sub.2 -adrenergic receptor subtype mRNAs in human tissues" Molecular Brain Research (1992) 16: 57-63.
Roemer, K. and Friedman, T., Concepts and strategies for human gene therapy, Eur. J. Biochem. (1992) 208.
Schwinn, D.A., et al., Molecular Cloning and Expression of the cDNA for a Novel .alpha..sub.1 -Adrenergic Receptor Subtype. J. Biol. Chem. (1990) 265:8183-8189.
Schwinn, D.A., and Lomasney, J.W., Pharmacologic characterization of cloned .alpha..sub.1 -adrenoceptor subtypes: selective antagonists suggest the existence of a fourth subtype, Eur. J. Pharmacol. (1992) 227(4): 433-436.
Voight, M.M., et al., Sequence of a rat brain cDNA encoding an alpha-1B adrenergic receptor. Nucleic Acids Res. (1990) 18(4): 1053.
Wagner, E., et al., Transferrin polycation DNA complexes: The effect of polycations on the structure of the complex and DNA delivery to cells, Proc. Natl. Acad. Sci. USA (1991) 88: 4255-4259.
Waspe, L.E., et al., The cardiac beta-myosin heavy chain isogene is induced selectively in alpha.sub.1 -adrenergic receptor-stimulated hypertrophy of cultured rat heart myocytes, J. Clin. Invest. (1990) 85(4): 1206-1214.
Weinshank, R.L., et al., "Human serotonin 1D receptor is encoded by a subfamily of two distinct genes: 5-HT.sub.1D.alpha. and 5-HT.sub.1D.beta. " Proc. Natl. Acad. Sci. USA (1992) 89: 3630-3634.
Yakubov, L.A., et al., Mechanism of oligonucleotide uptake by cells: Involvement of specific receptors?, Proc. Natl. Acad. Sci. USA 1989 86:6454-6458.
Yang-Feng,, T.L., et al., "Chromosomal organization of adrenergic receptor genes" Proc. Natl. Acad. Sci. USA (1990) 87: 1516-1520.
Bard Jonathon A.
Forray Carlos
Weinshank Richard L.
Elliott George C.
Synaptic Pharmaceuticals Corporation
Wang Andrew
White John P.
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