Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1987-03-11
1989-12-12
Teskin, Robin
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 43525233, 435320, 536 27, 935 11, 935 29, 935 39, 935 44, 935 56, 935 60, 935 73, C12P 2100, C12P 2102, C12N 500, C12N 1500
Patent
active
048867489
ABSTRACT:
The whole DNA sequence coding for flagellin of E. coli (hag gene) cloned in phage .lambda. was determined. The hag gene is introduced into pBR322 and the region of the hag gene concerning the antigencity of flagella is lacked, into which linkers are inserted. Where the vector in which foreign DNA is inserted in the linker is introduced in E. coli forming no flagella, the E. coli can form flagella and possesses motility. The flagella comprises flagellin fused with foreign peptide encoded by the foreign DNA. Namely, the foreign peptide is excreted outside of bacteria. This system can be utilized in determination of epitope and preparation of antigen as well as excretion of peptide.
REFERENCES:
Szekely et al., J. Bact., vol. 155, pp. 74-81, 1983, "DNA Sequence Adjacent to Flagellar Genes and Evolution of Flagellar Phase Variation".
Kondoh et al., J. Bact, vol. 130, pp. 736-745, 1977, "Isolation and Characterization of Nondefective Transducing Lamda Bacteriophages Carrying Fla genes of Eschericha coli K-12".
Joys et al., J. Bio. Chem., vol. 260, pp. 15758-1576, 1985, "The Covalent Structure of the Phase-1 Flagellar Filament Protein of Salmonella Typhimurium and Its Comparison with Other Flagellins".
Wei et al., 1985, J. Mol. Biol., vol. 186, pp. 791-803, "Covalent Structure of Three Phase-1 Flagellar Filament Proteins of Salmonella".
Gill et al., J. Biol. Chem., vol. 258, pp. 7395-7401, 1983, "The Nucleotide Sequence of the M.sub.1 =28,500 Flagellin Gene of Caulobacter Crescentus".
Asaka Junichiro
Fujiwara Takashi
Fujiwara Tamio
Kondo Eiji
Kuwajima Goro
Shionogi & Co. Ltd.
Teskin Robin
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