DNA encoding enzyme, recombinant DNA and enzyme, transformant, a

Details DNA encoding enzyme, recombinant DNA and enzyme, transformant, a DNA encoding enzyme, recombinant DNA and enzyme, transformant, a

1996-02-26

1998-02-10

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435200, 435201, 435276, C12N 924, C12N 926, C12N 102, C13J 100

Patent

active

057168130

ABSTRACT:
Disclosed are a DNA encoding an enzyme which releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher, recombinant DNA and enzyme, transformant, and their preparations and uses. These facilitate the industrial-scale production of trehalose with a relative easiness and low cost, and trehalose thus obtained can be satisfactorily used in a variety of food products, cosmetics and pharmaceuticals.

REFERENCES:
patent: 4521252 (1985-06-01), Miyake et al.
patent: 5455168 (1995-10-01), Maruta et al.
patent: 5472863 (1995-12-01), Maruta et al.
E. M. Southern, Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis, J. Mol. Biol., vol. 98, pp. 503-517, 1975.
Enzyme Kinetics of Amylases and Related Enzymes, Handbook of Amylases and Related Enzymes, pp. 1-9, 1988.
U. K. Laemmli, Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4, Nature, vol. 227, pp. 680-685, 15 Aug. 1970.
J. Sambrook et al, Molecular Cloning a Laboratory Manual, Second Edition, published by Cold Spring Harbor Laboratory Press, 1989, pp. 9.47-9.58 and 11.45-11.54.
Maruta et al., Biochimica et Biophysica Acta, "Cloning and Sequencing of Trehalose Biosynthesis Genes from Arthrobacter sp. Q36", No. 1289, 1996, pp. 10-13.

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