DNA encoding enzyme, recombinant DNA and enzyme, transformant, a

Details DNA encoding enzyme, recombinant DNA and enzyme, transformant, a DNA encoding enzyme, recombinant DNA and enzyme, transformant, a

1996-09-16

1999-02-16

Lau, Kawai

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435201, C12N 924, C12N 926

Patent

active

058719946

ABSTRACT:
A DNA encoding an enzyme, which forms non-reducing saccharides having trehalose structure as an end unit from amylaceous saccharides having a degree of glucose polymerization of 3 or higher, enables an industrial-scale production of a recombinant enzyme with such enzyme activity. Non-reducing saccharides obtainable by the recombinant enzyme can be used in a variety of food products, cosmetics, pharmaceuticals and feeds because of their substantial non-reducibility, mild and high-quality sweetness, adequate viscosity, and moisture-retaining ability.

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E.M. Southern, "Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis", J. Mol. Biol., vol. 98, pp. 503-517, 1975.

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