DNA encoding enzyme, recombinant DNA and enzyme, transformant, a

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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4353201, 4352523, 43525233, 536 232, C12N 924, C12N 121, C12N 1563, C07H 2104

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active

058719938

ABSTRACT:
A DNA encoding an enzyme, which forms non-reducing saccharides having trehalose structure as an end unit from amylaceous saccharides having a degree of glucose polymerization of 3 or higher, enables an industrial-scale production of a recombinant enzyme with such enzyme activity. Non-reducing saccharides obtainable by the recombinant enzyme can be used in a variety of food products, cosmetics, pharmaceuticals and feeds because of their substantial non-reducibility, mild and high-quality sweetness, adequate viscosity, and moisture-retaining ability.

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E.M. Southern, Detection of Specific Sequences Among DNA Fragments . . . Electrophoresis, J.Mol.Biol., vol. 98, pp. 503-517, 1975.
Alberts, B. et al. Molecular Biology of the Cell, Second Edition. New York: Garland Publishing. 1989, pp. 258-262, 265 and 266, 1989.
Stratagene. 1991 Product Catalog. pp. 220-221, 1991.

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