DNA encoding enzyme, recombinant DNA and enzyme, transformant, a

Details DNA encoding enzyme, recombinant DNA and enzyme, transformant, a DNA encoding enzyme, recombinant DNA and enzyme, transformant, a

1995-03-07

1996-09-17

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4352523, 4353201, 536 232, C12N 924, C12N 120, C12N 1500, C07H 2104

Patent

active

055567819

ABSTRACT:
Disclosed are a DNA encoding an enzyme which releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher, recombinant DNA and enzyme, transformant, and their preparations and uses. These facilitate the industrial-scale production of trehalose with a relative easiness and low cost, and trehalose thus obtained can be satisfactorily used in a variety of food products, cosmetics and pharmaceuticals.

REFERENCES:
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patent: 5455168 (1995-10-01), Maruta et al.
patent: 5472863 (1995-12-01), Maruta et al.
E. M. Southern, Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis, J. Mol. Biol., vol. 98, pp. 503-517, 1975.
Enzyme Kinetics of Amylases and Related Enzymes, Handbook of Amylases and Related Enzymes, pp. 1-9, 1988.
U. K. Laemmli, Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4, Nature, vol. 227, pp. 680-685, 15 Aug. 1970.
J. Sambrook et al, Molecular Cloning a Laboratory Manual, Second Edition, published by Cold Spring Harbor Laboratory Press, 1989.
Sinnott (1990) Chemical Reviews 90(7): 1171-1202.

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