Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1993-07-23
1995-11-07
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 694, 435 701, 435 703, 435 711, 435 712, 4352402, 4352523, 4351723, 4353201, 536 235, 536 2351, 536 231, 536 243, 536 2431, 536 2433, 935 11, 935 13, 935 24, 935 34, 935 56, 935 70, C12N 1512, C12N 1516, C12N 1563, C07K 14435
Patent
active
054647576
ABSTRACT:
A Corticotropin Releasing Factor-binding protein (CRF-BP) is isolated and purified sufficient to provide AA sequence data from which the DNA is obtained, which is then used to produce the protein recombinantly. The CRF-BP is useful for modulating the biological activity of CRF, such as reducing the high ACTH levels in mammals caused by excess CRF. The CRF-BP or fragments thereof and/or antibodies to the proteins may be employed in diagnostic assays to determine the levels of CRF, CRF-BP and the ratio of CRF/CRF-BP in a vascular fluid sample. The DNA or subsequence thereof can be used as probes for genetic material in certain assays. The anti-CRF-BP antibodies are also useful to purify the CRF-BP protein and to modulate the biological effect of CRF-BPs proteins.
REFERENCES:
Behan, et al., "The human plasma CRF-carrier protein: its isolation using affinity chromatography", J. Physiological Society, p. 101P, Dec. 1987.
Behan, et al., "Isolation of the CRH-Binding Protein from Human Plasma using Affinity Chromatography", Colloque de la Societe de Neuroendocrinologie Experimentale, Sep. 1988.
Behan, et al., "Isolation of the CRH-Carrier Protein from Human Plasma Using Affinity Chromatography", 8th International Congress of Endocrinology, Dec. 1987.
Potter, et al., "The central distribution of a corticotropin-releasing factor (CRF)-binding protein predicts multiple site and modes of interaction with CRF", Proc. Natl. Acad. Sci. USA, vol. 89, pp. 4192-4196, May 1992.
Potter, et al., "Cloning and characterization of the cDNAs for human and rat corticotropin releasing factor-binding proteins", Nature, vol. 349, pp. 423-426, Jan. 1991.
Suda, et al., "Corticotropin-Releasing Factor-Binding Protein is a Glycoprotein", Biochem. & Biophysical Research Communications, vol. 165, No. 2, pp. 703-707, Dec. 15, 1989.
Linton, et al., "Corticotropin-Releasing Hormone (CRH)-Binding Protein: Reduction in the Adrenocorticotropin-Releasing Activity of Placental but not Hypothalamic CRH", Journal of Clinical Endocrinology & Metabolism, vol. 70, No. 6, pp. 1574-1580, Nov. 1989.
Behan, et al., "Isolation of the human plasma corticotrophin-releasing factor-binding protein", Journal of Endocrinology, vol. 122, No. 1, pp. 23-31, Jul. 1989.
Linton, et al., "A Specific Carrier Substance for Human Corticotrophin Releasing Factor in Late Gestational Maternal Plasma which could Mask the ACTH-Releasing Activity", Clinical Endocrinology, 28, pp. 315-324, 1988.
Aebersold, et al., "Internal amino acid sequence analysis of proteins separated by one-or two-dimensional gel electrophoresis after in situ protese digestion on nitrocellulose", Proc. Natl. Acad. Sci. USA, vol. 84, pp. 6970-6974, Oct. 1987.
Behan, et al., "Cloning and Structure of the Human Corticotrophin Releasing Factor-Binding Protein Gene" [CRHBP], Genomics, 16, 63-68 (1993).
Linton, et al., "Corticotrophin Releasing Hormone Binding Protein [CRHBP]: Plasma levels decrease during the third trimester of human pregnancy", J. Clin. Endocr. Metab., 76, 260-262 (1993).
Suda et al., "Corticotropin-releasing factor-binding protein is a glycoprotein," Biochem. and Biophys. Research Comm., 165(2):703-707 (1989).
Potter et al., "Cloning and characterization of the cDNAs for human and rat corticotropin releasing factor-binding protein," Nature, 349:423-426 (1991).
Linton et al., "A specific carrier substance for human corticotrophin releasing factor in late gestational maternal plasma which could mask the acth-releasing activity," Clinical Endocrinology, 28:315-324 (1988).
Behan Dominic P.
Fischer Wolfgang H.
Linton Elizabeth A.
Lowry Philip J.
Potter Ellen
Patterson Jr. Charles L.
Prouty Rebecca
The Salk Institute for Biological Studies
University of Reading
LandOfFree
DNA encoding CRF binding protein does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with DNA encoding CRF binding protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA encoding CRF binding protein will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-196706