DNA encoding clostridium perfringens alpha-toxin peptides

Details DNA encoding clostridium perfringens alpha-toxin peptides DNA encoding clostridium perfringens alpha-toxin peptides

1996-10-04

1998-12-22

McKelvey, Terry A.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

435243, 4353201, 435410, 536 231, 536 234, 536 237, C12N 510, C12N 100, C12N 1511, C12N 1563

Patent

active

058518274

ABSTRACT:
The present invention provides novel peptides and vaccines containing them capable or inducing production of antibodies directed against Clostridium perfringens alph-toxin (CPa) in animals to which the) arc administered and thereby providing pro-phylazis against infection by Clostridium pefringens and/or the alpha-toxin itself. Particularly the present invention provides such a vaccine that is relatively safe and simple to produce. e.g. by genetic engineering means. Preferred peptides comprise theo amino acid sequence of Clostridium perfringens alpha-toxin from amino acid 247 to 370 but lack the epitopes necessary for phos-pholipase C and/or sphingomyelin hydrolysing activity round between amino acids 1 to 240 of that sequence. Further provided are antisera and antibodies raised to the peptides and vaccines of the present invention, and particularly monoclonal antibodies and hybridoma cell lines for their production.

REFERENCES:
patent: 4877612 (1989-10-01), Berger et al.
patent: 5200318 (1993-04-01), Rabin et al.
Fudenberg, H. H., MD., et al. (Editors), "Experimental Immunotherapy", Basic & Clinical Immunology, 3.sup.rd Edition, Chapter 44, Lange Medical Publications, pp. 722-736, 1994.
Jacob, Immunobiology of Proteins and Peptides V Vaccines, edited by Atassi, Plenum Press, New York, New York, pp. 9-16 (1989).
Infection and Immunity, vol. 59, No. 12, Dec. 1991, pp. 4338-4342 Logan et al, "Epitope mapping of the alpha-toxin of Clostridium perfringens".
Infection and Immunity vol. 59, No. 5, May 1991 pp. 1872-1874 Titball et al "Hemolytic and spinomyelinase activities of Clostridium perfringens alpha-toxin are dependent on a domain in homologous to that of an enzyme from the human arachidonic acid pathway".
Infection and Immunity vol. 57, No. 2, Feb. 1989 pp. 367-376 Titball et al "Molecular cloning and nucleotide sequence of the alpha-toxin (phospholipase C) of Clostridium perfringens".

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