DNA encoding chimeric fibroblast growth factors

Details DNA encoding chimeric fibroblast growth factors DNA encoding chimeric fibroblast growth factors

1994-01-05

1994-12-06

Hill, Jr., Robert J.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2351, 435 691, 435 694, 435 9132, 4351721, 4352523, 43525233, 4353201, 530399, C12V 1501, C12V 1518

Patent

active

053712064

ABSTRACT:
The present invention relates to novel chimeric fibroblast growth factors (FGF) wherein the alanine at amino acid 3 and serine 5 of native human recombinant basic fibroblast growth factor are replaced with glutamic acid. The N-terminus sequence of the present chimeric FGFs identify homology with that of human acidic fibroblast growth factor. The mitogenic properties of the native human recombinant basic FGF are exhibited by the present chimeric FGFs, and they are efficiently expressed in E. coli at significantly greater yields that previously reported. Novel variants of this new glu.sup.3,5 basic fibroblast growth factor, such as those in which cysteine 78 and cysteine 96 are replaced, e.g., with serine or other amino acids, to produce stabilized versions of the glu.sup.3,5 basic FGF and eliminate disulfide scrambled forms, are also described.

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patent: 4588585 (1986-05-01), Mark et al.
patent: 4959314 (1990-09-01), Mark et al.
Bollag et al., J. Mol. Biol., 218:493-494 (1991).
Seno et al., Biochem. Biophys. Res. Comm., 151(2):701-708 (1988).

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