Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-10-03
1996-12-10
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 237, 536 234, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104
Patent
active
055829902
ABSTRACT:
Disclosed and claimed are isolated nucleic acid molecules, such as DNA encoding Borrelia burgdorferi OspA, vectors containing the nucleic acid molecules, and methods for diagnosing Borrelia burgdorferi infection employing such nucleic acid molecules. The isolated nucleic acid molecule can be an isolated DNA molecule encoding the 31 kD OspA protein of New York strain B31. The isolated nucleic acid molecule also can be an isolated DNA molecule encoding Borrelia burgdorferi OspA and a signal peptide which contains an amino acid recognition sequence. The recognition sequence can be L-z-z-C, where each z independently designates a small, neutral amino acid, such as isoleucine or alanine. The recognition sequence can also be L-I-x-C where x is a non-charged amino acid residue, such as alanine. Further, the isolated nucleic acid molecule can be an isolated DNA molecule encoding Borrelia burgdorferi OspA and which includes a 5'-flanking region containing at least one promoter sequence for expression of the OspA. And additionally, the isolated nucleic acid molecule can be an isolated DNA molecule encoding Borrelia burgdorferi OspA as a fusion polypeptide containing the OspA.
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Sanger et al,
Barbour Alan G.
Bergstrom Sven
Magnarelli Louis A.
Arthur Lisa B.
Jones W. Gary
Kowalski Thomas J.
Symbicom Aktiebolag
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