DNA encoding (ASP 113) and (LYS 46, ASP 113) thaumatin I

Details DNA encoding (ASP 113) and (LYS 46, ASP 113) thaumatin I DNA encoding (ASP 113) and (LYS 46, ASP 113) thaumatin I

1988-05-02

1995-11-07

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

536 236, C12N 119, C12N 1529

Patent

active

054647703

ABSTRACT:
Genes are disclosed which are capable of directing the synthesis in a selected host microorganism of two thaumatin I analogues both of which have the amino acid sequence of natural thaumatin I including an aspartate amino acid residue in the 113th position from the amino terminal end of the polypeptide and one of which additionally has a lysine amino acid residue substituted for asparagine in the 46th position from the amino terminal end.

REFERENCES:
patent: 4657857 (1987-04-01), Ednes et al.
patent: 4666839 (1987-05-01), Souza
patent: 4891316 (1990-01-01), Verrips
Gouy et al. "Codon Usage in Bacteria: Correlation with Gene Expressivity" Nucleic Acids Research, 10, 7054-7074 (1982).
Bennetzer et al. "Codon Selection in Yeast". The Journal of Biological Chemistry, 257, 3026-3031 (1982).
Edens, et al. "Cloning of cDNA Encoding the Sweet-tasting Plant Thaumatin and its Expression in Escherichia coli", Gene, 18, 1-12 (1982).
Ivengar, et al., "The Complete Amino Acid Sequence Of The Sweet Protein Thaumatin I", Eur. J. Biochem. 96, pp. 193-204 (1979).
Science News, vol. 127, p. 186, Mar. 23, 1985.
Edens and van der Wel, Trends in Biotechnology, vol. 3, No. 3, pp. 61-64 (Mar. 1985).
Sensory Properties of Foods, Birch, G. C., Brennan, J. G. and Parker, L. J., eds., pp. 129-149 (1977) (Higginbotham).

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