Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-10-15
1996-01-30
Draper, Garnette D.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 691, 4353201, 4352402, 4352523, 43525411, 536 235, C12N 1512, C12N 500, C12N 111, G01N 3368
Patent
active
054879760
ABSTRACT:
The present invention provides for the isolation of genomic DNA fragments from Drosophila melanogaster encoding conserved amino acid sequence elements unique to the ligand-gated chloride channel gene family. A polymerase chain reaction (PCR)-based homology probing strategy was utilized to isolate the genomic DNA fragments. Using the PCR strategy, three discrete amplified sequence elements designated LCCH1, LCCH2 and LCCH3, were isolated, LCCH2 and LCCH3 being novel. In another aspect of the present invention, there is provided the cloning and characterization of a cDNA encoding a novel member of the ligand-gated chloride channel gene family of insects isolated from Drosophila melanogaster. The cDNA corresponds to the LCCH3 genomic sequence of the invention.
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Henderson Joseph E.
Knipple Douglas C.
Soderlund David M.
Cornell Research Foundation Inc.
Draper Garnette D.
Fitzgerald David L.
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