DNA encoding .alpha.-glucosidase and method of producing same by

Details DNA encoding .alpha.-glucosidase and method of producing same by DNA encoding .alpha.-glucosidase and method of producing same by

1993-03-22

1996-08-27

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435201, 435243, 4352401, 4352523, 43525411, 536 232, C12N 928, C12N 926, C12N 1556, C12N 1563, C12N 510, C12N 100

Patent

active

055500463

ABSTRACT:
A protein having an .alpha.-glucosidase activity, wherein its N-terminal amino acid sequence is the sequence depicted in the Sequence Listing of the present invention at Sequence No: 1; a DNA having the genetic information of the protein having an .alpha.-glucosidase activity; a recombinant vector containing said DNA; a transformant transformed with said vector; and production of .alpha.-glucosidase comprising culture of said transformant in a medium so as to grow .alpha.-glucosidase, and harvesting said .alpha.-glucosidase. According to the present invention, .alpha.-glucosidase without contamination of amylase can be easily produced in high yields and in large amounts by genetic engineering.

REFERENCES:
patent: 4493893 (1985-01-01), Mielenz et al.
Enzyme Nomenclature 1984. New York:Academic Press, pp. 310-311.
Kapetzi et al. (1989) Yeast 5:11-24.
Takii et al. (1992) App. Microbiol. Biotechnol. 38 243-247.
Suzuki et al (1984) Biochin Biophys Acta 787 281-289.
McLaughlin J. R. et al. (1981) Journal of Biological Chemistry 256: 11283-11291.
Jordan A. et al. (1994) Journal of Bacteriology 176: 3420-3427.
Suzuki et al., "Production of Extracellular .alpha.-Glucosidase by a Thermophilic Bacillus Species", Applied and Environmental Microbiol., 31, 6, 807-812 (1976).
American Type Culture Collection, Catalogue of Bacteria and Bacteriophages, Gherna et al., eds., 17th ed., p. 33 (1989).
Suzuki et al., "Assign," Biochimica et Biophysica Acta, 787, 281-289 (1984) .

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