DNA encoding a thermostable DNA polymerase

Details DNA encoding a thermostable DNA polymerase DNA encoding a thermostable DNA polymerase

1998-05-06

2000-11-07

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

C12N 900

Patent

active

061435368

ABSTRACT:
A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3'-5' exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.

REFERENCES:
patent: 5352778 (1994-10-01), Comb
patent: 5491086 (1996-02-01), Gelfand
patent: 5500363 (1996-03-01), Comb
patent: 5688779 (1997-11-01), Sun et al.
M. Kitabayashi et al. KOD DNA polymerase: application for fast and accurate PCR, FASEB Journal, vol. 10(6), No. 1423, 1996.
Database EMBL, DNA-Sequence data library, Hiedelberg, BRD Creation AC D29671, XP002017309, Apr. 30, 1994.
Morikawa et al. Purification and characterization of a thermostable thiol protease from a newly isolated hyperthermophilic pyrococcus sp. Appl. Enron. Microbiol. vol. 60(12), pp. 4559-4566, 1994.
Knog et al. Charaterization of a DNA polymerase from the hyperthermophile Archaea Thermococcus litoralis, The Journal of Biological Chemistry vol. 268(3), pp. 1965-1975, 1993.
Kakihara et al., Abstract No. 469, p. 168, 1993 General Meeting of Japan Association of Biotechnology, publ. Nov. 10, 1993, presented Dec. 8, 1993.
Nishioka et al., Abstract No. 354, p. 95, 1994 General Meeting of Japan Association of Biotechnology, publ. Oct. 10, 1994, presented Nov. 29, 1994.
Kakihara et al., Abstract No. 2Za9, Nippon Nogeikagaku Kaishi, 68:3, p. 146, publ. Mar. 5, 1994.
Morikawa et al., Applied and Environmental Microbiology, 60:12, Dec. 1994, pp 4559-4566.
Mullis et al., Cold Spring Harbor Symposia on Quantitative Biology, vol. 51, pt. 1, pp 263-273, 1986.
A. Skerra, Nucl. Acids Res., 20:14, pp 3551-3554, 1992.

Affiliated with

Also associated with

Rating

DNA encoding a thermostable DNA polymerase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with DNA encoding a thermostable DNA polymerase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA encoding a thermostable DNA polymerase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1639411