DNA encoding a Streptomyces endochitinase 56 signal peptide

Details DNA encoding a Streptomyces endochitinase 56 signal peptide DNA encoding a Streptomyces endochitinase 56 signal peptide

1990-02-01

1993-02-23

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 691, 435 712, 435169, 4351721, 4351723, 4352404, 4352523, 43525233, 43525234, 43525235, 4353201, 536 232, 536 237, 536 241, 935 6, 935 9, 935 14, 935 22, 935 29, 935 48, C12N 1574, C12N 1552, C12N 121

Patent

active

051889617

ABSTRACT:
Engineered DNA encoding a pre-protein which comprises a Streptomyces chitinase signal sequence and a desired mature protein. Preferably, the signal sequence is from the S. plicatus endochitinase gene. The engineered DNA is included in a host organism causing the host to produce and secrete the mature protein. Where the mature protein is a chitinase, the invention is used in methods of controlling chitinase-sensitive pests.

REFERENCES:
patent: 4751081 (1988-06-01), Suslow et al.
Fishman et al., 1985, J. Bacteriol., vol. 161:199-206.
Trumbly et al., J. Biol. Chem., vol. 26, #9, (1985), pp. 5683-5690.
Ghrayeb et al., EMBO, vol. 3, #10, (1984), pp. 2437-2442.
Trimble et al., J. Biol. Chem., vol. 254, #19, (1979), pp. 9708-9711.
Fuchs et al., Applied and Environ. Microbiol., 51(3):504-509 (1986).
Trimble et al., Methods in Enzymology, (1982), pp. 604, 1st paragraph.

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