Chemistry: molecular biology and microbiology – Vector – per se
Patent
1995-06-05
1999-02-09
Myers, Carla J.
Chemistry: molecular biology and microbiology
Vector, per se
536 235, 536 2431, 536 2433, 935 8, 935 9, 935 21, C07H 2104, C12N 1563
Patent
active
058693300
ABSTRACT:
The present invention relates to DNA encoding Acrp30, of vertebrate (e.g., mammalian) origin, and particularly of human and rodent origin. The present invention further relates to isolated, recombinantly produced or synthetic DNA which hybridizes to the nucleotide sequences described herein and RNA transcribed from the nucleotides sequence described herein. In addition, the invention relates to expression vectors comprising DNA encoding Acrp30, which is expressed when the vector is present in an appropriate host cell. The invention further relates to isolated, recombinantly produced or synthetic mammalian Acrp30 of vertebrate (e.g., mammalian) origin, and particularly of human and rodent origin. Also encompassed by the present invention is an inhibitor or enhancer of Acrp30. The present invention further relates to a method of identifying inhibitors or enhancers of Acrp30. Isolation of Acrp30 makes it possible to detect Acrp30 or adipocytes in a sample (e.g., test sample). In addition, the present invention relates to a method of regulating the energy balance (e.g., nutritional status) of a mammal by administering to the mammal an inhibitor or enhancer of the Acrp30.
REFERENCES:
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Scherer, P.E., et al., "A Novel Serum Protein Similar to C1q, produced Exclusively in Adipocytes", Journal of Biological Chemistry, 270:26746-26749 (1995).
Maeda, K., et al., cDNA Cloning and Expression of a Novel Adipose Specific Collagen-like Factor, apM1 (Adipose Most Abundant Gene Transcript 1), Biochemical & Biophysical Research Communications, 221:286-289 (1996).
EMBL, accession No. D45371, Sequence Reference g871886, Jun. 28, 1995, Human apM1 mRNA for GS3109.
EMBL, accession No. U37222, Sequence Reference g1051267, Nov. 7, 1995, Mus musculus 30 kDa adipocyte complement-related protein Acrp30 mRNA.
EMBL, accession No. S23297, Sequence Reference S22243, Oct. 7, 1994, chicken mRNA complement C1q carboxy-terminal homology.
Maeda et al., Genbank Sequence Listing, 1995.
Lodish Harvey F.
Scherer Philipp E.
Myers Carla J.
Whitehead Institute for Biomedical Research
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