Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1993-09-10
1995-04-11
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
4352528, 4353201, 435194, 536 232, 935 10, 935 14, C12N 912, C12N 1500, C12N 120
Patent
active
054057744
ABSTRACT:
A purified thermostable enzyme is derived from the bacterium Thermus species sps17. The enzyme has DNA polymerase activity, reverse transcriptase activity, and optionally, 5'.fwdarw.3' exonuclease activity. The enzyme can be native or recombinant, and can be used with selected primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
REFERENCES:
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 4965188 (1990-10-01), Mullis et al.
Chien et al., Sep. 1976, "Deoxyribonucleic Acid Polmerase from the Extreme Thermophile Thermus Aquaticus" J. Bacteriology 127(3):1550-1557.
Kaledin et al., 1980, "Isolation and Properties of DNA Polymerase from Extremely Thermophilic Bacterium Thermus Aquaticus YT1" Biochem. 45(4):494-501.
Suggs et al., Nov. 1981, "Use of Synthetic Oligonucleotides as Hybridization Probes: Isolation of Cloned cDNA Sequences for Human Beta-2-Microglobulin" Proc. Natl. Acad. Sci. USA 78(11):6613-6617.
Young and Davis, Mar. 1983, "Efficient Isolation of Genes by Using Antibody Probes" Proc. Natl. Acad. Sci. USA 80:1194-1198.
Bernad et al., 1989, "A Conserved 3'-5' Exonuclease Active Site in Prokaryotic and Eukaryotic DNA Polymerases" Cell 59:219-228.
Lawyer et al., 1989 "Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene From Thermus Aquaticus" J. Biological Chemistry 264(11)6427-6437.
Leavitt and Ito, Jun. 1989, "T5 DNA Polymerase: Structural-Fuctional Relationships to Other DNA Polymerases" Prod. Natl. Acad. Sci. USA 86:4465-4469.
Degryse et al., 1978, "A Comparative Analysis of Extreme Thermophilic Bacteria Belonging to the Genus Thermus" Arch. Microbiol. 117:189-196.
Microbiology, Fifth Ed., 1986, pp. 41-49, edited by M. J. Pelczal, Jr. et al. published by McGraw-Hill Book Company (New York) "The Characterization, Classification and Identification of Microorganisms".
Microbiology, Fourth Ed., 1990, pp. 11-19, edited by B. D. Davis et al., published by J. B. Lippincott Company (Philadelphia) "Evolution of Microbiology and of Microbes".
"Biochemical Taxonomy of the Genes Thermus", published in: Microbiology of Extreme Environments and its Potential for Biotechnology pp. 82, et seq., eds. M. S. DaCosta, J. C. Durate and R. A. D. Williams, a volume of The Proceedings of the Federation of European Microbiological Societies Symposium held in Troia, Portugal, 18-23 Sep. 1988, published by Elsevier Applied Science (London and New York).
Gelfand, D. H, PCR Technology, Principles and Applications for DNA Amplification, Chapter 2, Entitled "Taq DNA Polymerase" pp. 17-22 (1989), Ed. by H. A. Erlich.
Abramson Richard D.
Gelfand David H.
Greenfield I. Lawrence
Gould George M.
Hendricks Keith D.
Hoffmann-La Roche Inc.
Sias Stacey R.
Tramaloni Dennis P.
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