DNA encoding a KEX2 endoprotease without a C-terminal hydrophobi

Details DNA encoding a KEX2 endoprotease without a C-terminal hydrophobi DNA encoding a KEX2 endoprotease without a C-terminal hydrophobi

1992-07-22

1993-08-10

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4353201, 435 691, 435224, 935 14, 935 10, 536 232, 536 2374, C12N 120, C12N 960

Patent

active

052348300

ABSTRACT:
A KEX2 endoprotease produced by a recombinant DNA technique, a KEX2 endoprotease shortened at the C-terminal of a native enzyme and still containing a C-terminal hydrophobic region, a soluble KEX2 endoprotease without a C-terminal hydrophobic region; DNA's coding for the above-mentioned enzymes; expression plasmids containing the DNA; hosts transformed with the plasmid; a process for production of the above-mentioned enzymes using the transformed host; and a process for production of biologically active polypeptide using the above-mentioned enzyme.

REFERENCES:
Fuller et al., "The Saccharomyces Cerevisiae KEX2 Gene, Required for Processing Prepro-.alpha.-Factor, Encodes a Calcium-Dependent Endopeptidase that Cleaves After Lys-Arg and Arg-Arg Sequences," Microbiology, pp. 273-278, 1986.
Julius et al., "Isolation of the Putative Structural Gene for the Lysine-Argine-Cleaving Endopeptidase Required for Processing of Yeast Prepro-.alpha.-Factor," Cell, 37, pp. 1075-1089, 1984.
Wickner et al, "Two chromosomal Genes Required for Killing Expression in Killer Strains of Saccharomyces Cerevisiae," Genetics, 82, pp. 429-442, 1976.
Mizuno et al., "A Membrane-Bound, Calcium-Dependent Protease In Yeast .alpha.-Cell Cleaving on the Carboxyl Side of Paired Basic Residues," BBRC, 144, pp. 807-814, 1987.
Leibowitz et al., "A Chromosomal Gene Required for Killer Plasmid Expression, Mating, and Spore Maturation in Saccharomyces Cerevisiae," PNAS, 73, pp. 2061-2065, 1976.
Bathurst et al., "Yeast KEX2 Protease Has the Properties of a Human Proalbumin Converting Enzyme," Science, 235, pp. 348-350, 1987.
Fuller et al., "Enzymes Required for Yeast Prohormone Processing," Ann. Rev. Physiol., pp. 345-362, 1988.
Thomas et al., "Yeast KEX2 Endopeptidase Correctly Cleaves a Neuroendocrine Prohormone In Mammalian Cells" Science, 241, pp. 226-230, 1988.
Mizuno et al., "Yeast KEX2 Gene Encodes an Endopeptidase Homologous to Subtilisin-Like Serine Protease," BBRC, 156, pp. 246-254, 1988.
R. S. Fuller et al., Journal of Cellular Biochemistry, Supplemental 12B, Jan. 30, 1988, p. 27, Abstract No. X015.

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