DNA encoding a human neuropeptide Y/peptide YY (Y2) receptor and

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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435 691, 435 703, 435 712, 4353201, 536 231, 536 235, C07H 2104, C12N 510, C12N 1512, C12N 1563

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active

055455492

ABSTRACT:
This invention provides an isolated nucleic acid molecule encoding a human Y2 receptor, an isolated protein which is a human Y2 receptor, vectors comprising an isolated nucleic acid molecule encoding a human Y2 receptors, mammalian cells comprising such vectors, antibodies directed to the human Y2 receptor, nucleic acid probes useful for detecting nucleic acid encoding human Y2 receptors, antisense oligonucleotides complementary to any sequences of a nucleic acid molecule which encodes a human Y2 receptor, pharmaceutical compounds related to human Y2 receptors, and nonhuman transgenic animals which express DNA a normal or a mutant human Y2 receptor. This invention further provides methods for determining ligand binding, detecting expression, drug screening, and treatment involving the human Y2 receptor.

REFERENCES:
Wahlestedt, C., et al. Neuropeptide Y Receptor Subtypes, Y1 and Y2. Ann. N. Y. Acad. Sci. 1990; 611:7-26 (Exhibit B).
Wahlestedt, C., et al. Indentification of Cultured Cells Selectively Expressing Y1-, Y2-, Or Y3-Type Receptors for Neuropeptide Y/Peptide YY. Life Sciences 1991; 50(4): PL-7 -PL-12 (Exhibit C).
Sheikh, S. P., et al. Binding of Monoiodinated Neuropeptide Y to Hippocampal Membranes and Human Neuroblastoma Cell Lines. J. Biol. Chem. 1989; 264(12):6648-6654 (Exhibit D).
Larhammar, D., et al. Cloning and Functional Expression of a Human Neuropeptide Y/Peptide YY Receptor of the Y1 Type. J. Biol. Chem. 1992; 267(16):10935-10938 (Exhibit E).
Wahlestedt et al Life Sciences 50 PL-7 -PL-12 (1991)
Kluxen et al PNAS 89 4618-4622 (1992).
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Inui et al. Ann. N. Y. Acad Sci. 611 350-352 (1990) Narray et al. Neurosci. Lett. 140 273-276 (1992).
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