DNA encoding a human 5-HT .sub.1F receptor and uses thereof

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 691, G01N 3353

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active

056521138

ABSTRACT:
This invention provides an isolated nucleic acid molecule encoding a human 5-HT.sub.1F receptor, an isolated protein which is a human 5-HT.sub.1F receptor, vectors comprising an isolated nucleic acid molecule encoding a human 5-HT.sub.1F receptors, mammalian cells comprising such vectors, antibodies directed to the human 5-HT.sub.1F receptor, nucleic acid probes useful for detecting nucleic acid encoding human 5-HT.sub.1F receptors, antisense oligonucleotides complementary to any sequences of a nucleic acid molecule which encodes a human 5-HT.sub.1F receptor, pharmaceutical compounds related to human 5-HT.sub.1F receptors, and nonhuman transgenic animals which express DNA a normal or a mutant human 5-HT.sub.1F receptor. This invention further provides methods for determining ligand binding, detecting expression, drug screening, and treatment involving the human 5-HT.sub.1F receptor.

REFERENCES:
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Amlaiky, N., et al. J. Biol. Chem. 1992; 267(28):19761-19764.
Leonhardt, S., et al. Detection of a Novel Serotonin Receptor Subtype (5-HT1E) in Human Brain: Interaction with a GTP-Binding Protein. J. Neurochemistry 1989; 53(2):465-471.
Adham, N., et al. Cloning of another human serotonin receptor (5-HT1F): A fifth 5-HT1 receptor subtype coupled to the inhibition of adenylate cyclasel Proc. Natl. Acad. Sci. U.S.A. 1993; 90:408-412.
Weisberg et al., Drug Development Research, 26(3): 225-234, 1992.
Schwab et al., Soc. Neurosci. Abstr., 15(1): 546, 1989, Abstract 222.9.

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