Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-07
1998-03-24
Walsh, Stephen
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 697, 435 711, 435358, 435365, 4352523, 4352542, 4352528, 4353201, 536 234, 536 2351, C12N 121, C12N 510, C12N 1511, C12N 1518
Patent
active
057311706
ABSTRACT:
Discoveries are disclosed that show particular aspects of recombinant DNA technology can be used successfully to produce hitherto unknown human keratinocyte growth factor (KGF) protein free of other polypeptides. These proteins can be produced in various functional forms from spontaneously secreting cells or from DNA segments .introduced into cells. These forms variously enable biochemical and functional studies of this novel protein as well as production of antibodies. Means are described for determining the level of expression of genes for the KGF protein, for example, by measuring mRNA levels in cells or by measuring antigen secreted in extracellular or body fluids.
REFERENCES:
Science, vol. 237, p. 1570, 1987. (Lewin).
Cell, vol. 50, p. 667, 1987 (Reeck et al.).
Van Brunt "Growth Factor Speed Wound Healing" Bio/Technology v 6(1) Jan. 1988.
Moore et al "Sequence, Topography and Protein Coding Potential of mouse int-2 . . . " EMBO J. V.S (5) pp. 919-924, 1986.
European Search Report (1pg).
Rubin, et al., "Purification and Characterization of a Newly Identified Growth Factor Specific for Epithelial Cells", Proc. Natl. Acad. Sci. USA, Vol. 86, pp. 802-806 (1989).
Finch, et al., "Human KGF is FGF-Related with Properties of a Paracrine Effector of Epithelial Cell Growth", Science, vol. 245, pp. 752-755 (1989).
Recombinant Basic Fibroblast Growth Factor Stimulates Wound Healing-impaired db/db Mice by Ryoji Tsuboi and Daniel B. Riftin, J. Exp. Med. vol. 172 Jul. 1990 245-251.
Basic Fibroblast Growth Factor Stimulation of Epidermal Wound Healing in Pigs by Patricia A. Hebda, Crine K. Klingbeil, Judith A. Abraham, and John C. Fiddes, The Society for Investigative Dermatology, Inc.
Effect of bFGF on the Inhibition of Contraction Caused by Bacteria, Brain D. Stenberg, Linda G. Phillips, James A. Hokanson, John P. Heggers and Martin C. Robson, Journal of Surgical Research 50, 47-50 (1991).
Expression of the FGF-related proto-oncogene int-2 during gastrulation and neurulation in the mouse, The FMBO Journal vol. 7 No. 3 pp. 691-695, 1988.
Two proto-oncogenes implicated in mammary carcinogenesis, int-1 and int-2, are independently regulated during mouse development, Proc. Natl. Acad. Sci. USA vol. 83, pp. 7806-7810, Oct. 1986.
Mesoderm-inducing properties of INT-2 and kFGF: two oncogene-encoded growth factors related to FGF, By G.D. Paterno, L.L. Gillespie, M.S. Dixon, J.M. Slack and J.K. Heath, Development 106, 79-83 (1989).
The Herarin-Binding (Fibroblast) Growth Factor Family of Proteins, by Wilson H. Burges and Thomas Maciag, Annul. Rev. Biochem. 1989 58.
Alan D. Stiles, et al; Reciprocal Autocrine and Paracrine Regulation of Growth of Mesenchymal and Alveolar Epithelial Cells From Fetal Lung; Experimental Lung Research 11:165-177 (1986).
Kwan Y. Chan & Richard H. Haschke; Epithelial-Stromal Interactions: Specific Stimulation of Corneal Epithelial Cell Growth in vitro by a Factor(s) from Cultured Stromal Fibroblasts; Exp. Eye Res. (1983) 36, 231-246.
Polypeptide Growth Factors: Robert James Isolation of Brain Fibroblast Growth Factor By Heparin-Sepharose Affinity Chromatography: Identity with Pituitary Fibroblast Growth Factor: Dennis ospodarowics, Jannie Cheng, Ge-Ming Lui, Andrew W. Baird, and Peter Bohlent.
Heparin Binds Endothelial Cell Growth Factor, The Principal Endothelial Cell Mitogen Cell Mitogen in Bovine Brain: Science vol. 225.
The Isolation And Purification of Two Antionic Endothelial Cell Growth Factors From Human Brain: Greg Conn and Victor B. Hatcher.
Heparin Sulphate Bound Growth Factors: A Mechanism For Stromal Cell Mediated Haemopoiesis: R. Roberts, J. Gallagher, E. Spooncer, T.D. Allen, F. Bloomfield & T.M. Dexter.
Autocrine and Paracrine Growth Stimulation of Cells derived from Human Skin Barbara A. Gilchrest, Rita L. Karassik, Leon M. WIlkins, Michael A. Vrabel, and Thomas Maciag.
Balb and Kristen Murine Sarcoma Viruses Alter Growth And Differentiation of EGF-Dependent Balb/c Mouse Epidermal Keratinocyte Lines.
SV40 T Antigen Induction And Transformation In Human Fibroblast Cell Strains Stuart A. Aaronson and George J. Todaro.
Improved Tools For Biological Sequence Comparison: William R. Pearson and David J. Lipman.
Aaronson Stuart A.
Finch Paul W.
Rubin Jeffrey S.
Saoud Christine
The United States of America as represented by the Department of
Walsh Stephen
LandOfFree
DNA encoding a growth factor specific for epithelial cells does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with DNA encoding a growth factor specific for epithelial cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA encoding a growth factor specific for epithelial cells will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2287985