DNA encoding a cell membrane glycoprotein of a tick gut

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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4352401, 4352523, 43525233, 43525411, 4353201, 4352542, 536 235, C12N 100, C12N 115, C12N 121, C12N 510, C12N 1512, C12N 1563

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055873111

ABSTRACT:
The invention relates to antigens derived from ticks and to their purification. It also relates to genes encoding such antigens and to their cloning and expression from recombinant DNA molecules. Further, the invention describes the use of purified antigens and recombinant expression products having similar biological activity to those purified antigens to provide vaccines to protect cattle against tick infestation.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
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Johnston et al., "Immunization of Cattle Against Boophilus microplus Using Extracts Derived from Adult Female Ticks: Effects of Induced Immunity on Tick Populations", Inter. Journ. Parasitology, vol. 16, No. 1, 1984, pp. 27-34.
Brown et al., "Characterization of Tick Antigens Inducing Host Immune Resistance", Journ. Immun., vol. 133, No. 6, Dec. 1984, pp. 3319-3325.
Ackerman et al., "Artifical Immunity to Dermacentor variabilis (Acari: Ixodidae): Vaccination Using Tick Antigens.sub.1 ", J. Med. Entomol., vol. 17, No. 5, 1980, pp. 391-397.
McGowan et al., "Success of Tick Feeding on Calves Immunized with Amblyomma americanum (Acari: Ixodidae) Extract.sub.1 ", J. Med. Entomol., vol. 18, No. 4, 1981, pp. 328-332.
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George et al., "Acquistion and Expression of Resistance by Bos indicus and Bos indicus x Bos taurus Calves to Amblyomma Americanum Infestation", J. Parasit. vol. 71, No. 2, 1985, pp. 174-182.
S. K. Wikel., "Effects of Tick Infestation on the Plague-Forming Cell Response to a Thymic Dependent Antigen", Ann. Trop. Med. Parasit., vol. 79, No. 2, 1985, pp. 195-198.
S. K. Wilkel, "Resistance to Ixodid Tick Infestation Induced by Administration of Tickl-Tissue Culture Cells", Ann. Trop. Med. Parasit., vol. 79, No. 5, 1985, pp. 513-518.
Wikel et al., "Ixodid-Host Immune Interaction. Identification and Characterization of Relevant Antigens and Tick-Induced Host Immunosuppresion", Vet. Parasit., vol. 20, 1986, pp. 149-174.
Whelen et al., "Dot-Elisa Assessment of Guinea Pig Antibody Responses to Repeated Dermacentor andersoni Infestations", J. Parasit. vol. 72, No. 1, 1986, pp. 155-162.
Wikel et al., "Immunological Studies of Ixodid Tick-Host Interaction: Analysis of Immunogens", J. Toxicol. Toxin Rev. vol. 5, No. 2, 1986, pp. 145-160.
Sharp et al., "Chromatography and Generation of Specific Antisera to Synthetic Peptides from a Protective Boophilus Microplus Antigen", J. Chrom., vol. 512, 1990, pp. 189-202.
"Principles of Gene Manipulation", Blackwell Scientific Pibl., 1985, p. 13.

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