DNA encoding a bacterial protease for degrading gelatin and...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S006120, C435S252300, C435S320100, C435S325000, C536S023100, C536S023200

Reexamination Certificate

active

07871797

ABSTRACT:
The present invention relates to a protease having a molecular weight of 21,000±1,000 (by SDS-PAGE) and a specific protease activity capable of cutting at the Gly-Phe site of a peptide or protein comprising the amino acid sequence Pro-Gln-Gly-Phe-Gln-Gly-Pro or Pro-Ala-Gly-Phe-Ala-Gly-Pro or cutting at the Ser-Leu site of a peptide or protein comprising the amino acid sequence Gln-Thr-Gln-Ser-Leu-Val-Thr-Pro. The invention further provides DNA encoding this protease, as well as vectors containing this DNA, and a method for producing this protease by transforming a cell with this DNA.

REFERENCES:
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patent: 08-266276 (1996-10-01), None
patent: 2000-102381 (2000-04-01), None
patent: 2000-325095 (2000-11-01), None
patent: WO 2004/033668 (2004-04-01), None
Seffernick et al. (J. Bacteriology, vol. 183, pp. 2405-2410, 2001).
Wells, Biochemistry, vol. 29, pp. 8509-8517, 1990.
Lecroisey et al., FEBS Letters, (1975), vol. 59, No. 2, pp. 167-172.
Kabashima et al., Biochimica et Biophysica. Acta., (1999), vol. 1429, pp. 516-520.
Oqasawara et al., Biosci. Biotech. Biochem., (1997), vol. 61, No. 1, pp. 146-151.
Y. Kanayama, et al., “Purification and Properties of a New Type of Protease Produced byMicrobacterium liquefaciens” Biosci. Biotechnol. Biochem., 69 (5), pp. 916-921,2005.

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