DNA detector and DNA detection method

Details DNA detector and DNA detection method DNA detector and DNA detection method

1993-03-05

1994-05-24

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041828, 356344, G01N 2726, G01N 27447

Patent

active

053146026

ABSTRACT:
In a DNA detecting method wherein the fluorescence detection type electrophoresis apparatus is used, Texas Red is employed as a fluorophor which labels a DNA fragment, an He-Ne laser having an emission wavelength of 594 nm is used as an excitation light, the excitation light irradiates a line portion of the gel plate simultaneously, and the resulting linear fluorescent image is collected by a cylindrical lens and photodetected thereby providing an apparatus featuring a higher sensitivity, a smaller size and a lighter weight than conventional apparatuses.

REFERENCES:
patent: 3764512 (1973-10-01), Greenwood et al.
patent: 4938593 (1990-07-01), Morris et al.
patent: 5062942 (1991-11-01), Kambara et al.
patent: 5108179 (1992-04-01), Myers
H. Kambara et al., "Optimization of Parameters in a DNA Sequenator Using Fluorescence Detection", Bio/Technology, vol. 6, Jul. 1988, pp. 816-821.
L. Morrison et al., "Solution-Phase Detection of Polynucleotides Using Interacting Fluorescent Labels and Competitive Hybridization", Analytical Biochemistry 183, pp. 231-232, 1989.

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