DNA detector and DNA detection method

Details DNA detector and DNA detection method DNA detector and DNA detection method

2000-03-14

2000-12-05

Wallenhorst, Maureen M.

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

204603, G01N 2726, G01N 27447

Patent

active

061561770

ABSTRACT:
A method for analyzing samples includes the steps of introducing samples labeled with fluorophores into a first end of each of a plurality of electrophoresis lanes including a separation medium, a second end of each of the electrophoresis lanes being disposed in an optical cell filled with a buffer solution, applying an electric field to the electrophoresis lanes to separate the samples labeled with the fluorophores and cause the separated samples labeled with the fluorophores to migrate through the electrophoresis lanes into the buffer solution in the optical cell, exciting, with light from a light source, the fluorophores labeling the separated samples in the buffer solution in the optical cell to emit fluorescence in the buffer solution in the optical cell, and detecting, with a photodetector, the fluorescence emitted by the fluorophores labeling the separated samples in the buffer solution in the optical cell. The light source is selected from the group consisting of a He--Ne laser which emits light having a wavelength of 594 nm, a He--Ne laser which emits light having a wavelength of 632.8 nm, a YAG laser which emits light having a wavelength of 532 nm, and a semiconductor laser which emits light having a wavelength of 670 nm.

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L. Smith et al, "Fluorescence detection in automated DNA sequence analysis", Nature, pp. 674-679, Jun. 12, 1986.
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Y. Cheng et al, "Subattomole Amino Acid Analysis by Capillary Zone Electrophoresis and Laser-Induced Fluorescence," Science, vol. 242, pp. 562-564, Oct. 28, 1988.
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H. Swerdlow et al, "Capillary gel electrophoresis for DNA sequencing-Laser-induced fluorescence detection with the sheath flow cuvette" Journal of Chromatography, vol. 516, pp. 61-67, 1990, No month available.
Norman J. Dovichi et al, "Three DNA Sequencing Methods Using Capillary Gel Electrophoresis and Laser-Induced Fluorescence" Analytical Chemistry, vol. 63, No. 24, pp. 2835-2841, 1991, No month available.
L. Smith et al., "Fluorescence detection in automated DNA sequence analysis", Nature, vol. 321, Jun. 12, 1986, pp. 674-679.
H. Kambara et al., "Optimization of Parameters in a DNA Sequenator Using Fluorescence Detection", Bio/Technology, vol. 6, Jul. 1988, pp. 816-821.
Y. Cheng et al., "Subattomole Amino Acid Analysis by Capillary Zone Electrophoresis and Laser-Induced Fluorescence", Science, vol. 242, Oct. 28, 1988, pp. 562-564.
L. Morrison et al., "Solution-Phase Detection of Polynucleotides Using Interacting Fluorescent Labels and Competitive Hybridization", Analytical Biochemistry, vol. 183, 1989, pp. 231-232.
H. Swerdlow et al., "Capillary gel electrophoresis for rapid, high resolution DNA sequencing", Nucleic Acids Research, vol. 18, No. 6, 1990, pp. 1415-1419.
H. Swerdlow et al., "Capillary gel electrophoresis for DNA sequencing--Laser-induced fluorescence detection with the sheath flow cuvette", Journal of Chromatography, vol. 516, 1990, pp. 61-67.
H. Swerdlow et al., "Three DNA Sequencing Methods Using Capillary Gel Electrophoresis and Laser-Induced Fluorescence", Analytical Chemistry, vol. 63, No. 24, Dec. 15, 1991, pp. 2835-2841.

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