DNA detection system

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 7, 436501, 436 94, 935 77, G01N 3353, G01N 3300, G01N 33566, C12Q 168

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active

049786083

ABSTRACT:
Picogram amounts of DNA can be detected in a sample by the use of high affinity single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.

REFERENCES:
patent: 4493899 (1985-01-01), Smith
patent: 4493899 (1985-01-01), Smith et al.
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Voller et al., in Manual of Chemical Immunology, 3rd Ed., ASM, Washington, D.C., Editors, Rose, N. R. et al., pp. 99-109, 1986.
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"Large-Scale Overproduction and Rapid Purification of the Escherichia coli SSB Gene Product. Expression of the SSB Gene under .lambda. P.sub.L Control," by Lohman et al., Biochemistry, (1986), 25:21-25.
Smith et al., 1981, J. Lab. Clin. Med., 98:425-436.
Chase et al., Ann. Biochem., "Single-Stranded DNA Binding Proteins Required for DNA Replication", 55:103-136, (1986).
Kowalczykowski et al., Biochemistry, "DNA Binding Proteins", 17:425-429, (1978).
Krauss et al., Biochemistry, "Escherichia coli Single-Strand Deoxyribonucleic Acid Binding Protein: Stability, Specificity, and Kinetics of Complexes with Oligonucleotides and Deoxyribonucleic Acid", 20:5346-5352, (1981).
Kung et al., The Journal of Biological Chemistry, "Purification and Characterization of an .omega. Protein from Micrococcus luteus", 252:5398-5402, (1977).
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