DNA detection method

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...

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536402, 536387, 536350, 435 6, 435 7, 435177, 436501, C07, G01N 33566, C12Q 168, C12N 1102

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active

049636588

ABSTRACT:
Picogram amounts of DNA can be detected in a sample by the use of high-affinity, single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.

REFERENCES:
patent: 4493899 (1985-01-01), Smith et al.
Syvanen et al., Nuclear Acid Research, vol. 13, pp. 2789-1802, 1985.
Voller et al. in Manual of Clinical Laboratory Immunology, 3rd edition, Eds: Rose, Friedman & Fahey, 1986.
Tijssen Practice & Theory of Enzyme Immunoassays, vol. 15, Elsevier Press, 1985, see Chapter 11, pp. 221-230, 259-261.
Vogelstein et al., PNAS, U.S.A., 76:615-619, (1979).
Gellert, The Enzymes, 14:345-367, (1981).
Wang, The Enzymes, 14:332-343.
Chase et al., Ann. Rev. Biochem., 55:103-136, (1986).
Kowalczykowski et al., Biochemistry, 17:425-429, (1978).
Krauss et al., Biochemistry, 20:5346-5352, (1981).
Kung et al., The Journal of Biological Chemistry, 252:5398-5402, (1977).
DuClos et al., Journal of Immunological Methods, 88:185-192, (1986).
"Investigation of Complexes Formed Between Gene 32 Protein from Bacteriophase T-4 and Heavy-Atom-Modified Single-Stranded Polynucleotides Using Optical Detection of Magnetic Resonance," by Khamis et al., Biochemistry (1986), 25:5865-5872.
"Deoxyribonucleic Acid Topoisomerase I from Chicken Erythrocytes: Purification, Characterization, and Detection by a DNA Binding Assay," by Tricoli et al., Biochemistry (1983) 22:(8)2025-2031.
"Large-Scale Overproduction and Rapid Purification of the Escherichia coli SSB SSB Gene Product. Expression of the SSB Gene under .lambda. P.sub.L Control," by Lohman et al., Biochemistry (1986) 25:21-25.

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