DNA detection by color complementation

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, C12Q 168, C12P 1934

Patent

active

054895073

ABSTRACT:
A method of detecting a target DNA in a sample is provided which includes simultaneously amplifying the target DNA and one or more internal standard DNAs, labeling the target DNA with a first color-producing or color-absorbing label, and labeling each of the one or more internal standard DNAs with a different second color-producing or color-absorbing label, the first and second color-producing and color-absorbing labels being selected so that upon illumination of the sample a first color signal is produced whenever the target DNA is present and a second color signal is produced whenever the target DNA is absent.

REFERENCES:
patent: 3892530 (1975-07-01), Felix et al.
patent: 4495293 (1985-01-01), Shaffar
patent: 4581333 (1986-04-01), Kourilsky et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
"Polymerase Chain Reaction-Product Application Focus", Christian Oste, BioTechniques, vol. 6, No. 2, 1988.
"Length mutations in human mitochondrial DNA: direct sequencing of enzymatically amplified DNA", Lisa A. Wrischnik, Russell G. Higuchi, Mark Stoneking, Henry A. Erlich, Norman Arnheim and Allan C. Wilson, Nucleic Acids Research, vol. 15, No. 2, 1987.
Yamane et al, Nucleic Acids Research, Symposium Series No. 20, pp. 91-92 (1988).
Landegren et al, Science, vol. 242, pp. 229-237 (1988).
Smith et al. (1985) The Synthesis of Oligonucleotides Containing an Aliphatic Amino Group at the S.sup.1 Terminus Nuc. Acid. Res. 13(7)2399-2412.
Connolly B. (1985) Chemical Synthesis of Oligonucleotides Cont. a Freee Sulphydryl Group & Subsequent . . . Nu. Acid Res. 13 (12) 4483:4501.
Agrawal S (1986) Efficient Methods for Attaching Non-Radioactive Labels to The S.sup.1 of Synth. Oligo . . . Nu. Acid Res 14(15) 6227-45.

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