DNA denaturation method

Details DNA denaturation method DNA denaturation method

1993-03-30

1996-03-19

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C12Q 168

Patent

active

055003397

ABSTRACT:
Method for determining the nucleotide base sequence of a double-stranded closed circular DNA. The method includes heating a solution which includes the DNA in the presence of at least 20% glycerol and/or ethylene glycol to at least 80.degree. C. to provide denatured DNA, and then sequencing the denatured DNA.

REFERENCES:
patent: 4795699 (1989-01-01), Tabor et al.
patent: 4868115 (1989-09-01), Obayashi et al.
patent: 4962020 (1990-10-01), Tabor et al.
Oro et al, Influence of the Electrical Permittivity and Counter Ions Content of the Media on the Thermal Denaturation of DNA. Studia Biophysica (1987) 120:51-57.
Norden, B., et al. Structure of Strand Separated DNA in Different Environments Studied by Linear Dichroism, Biopolymeus (1979) 18:2323-2340 (Abstract).
Andersen et al., "A Fast and Simple Technique for Sequencing Plasmid DNA with Sequenase.RTM. Using Heat Denaturation," 13(5) BioTechniques 678, 1992.
Wallace et al., "A Set of Synthetic Oligodeoxyribonucleotide Primers for DNA Sequencing in the Plasmid Vector pBR322," 16 Gene 21, 1981.
Smith et al., "Sequence of the Gene for Iso-1-Cytochrome c in Saccharomyces cerevisiae," 16 Cell 753, 1979.
Bastia et al., "The Nucleotide Sequence Surrounding the Replication Terminus of R6K," 78 Proc. Natl. Acad. Sci. USA 2095, 1981.
Shon et al., "The Nucleotide Sequence of the Replication Origin .beta. of the Plasmid R6K," 257 J. Biol. Chem. 13823, 1982.
Straus and Zagursky, "In Vitro Production of Large Single-Stranded Templates for DNA Sequencing," 10(3) BioTechniques 376, 1991.
Mead and Kemper, "Chimeric Single-Stranded DNA Phage-Plasmid Cloning Vectors," Vectors: A Survey of Molecular Cloning Vectors and Their Uses, Butterworth Publishers, Massachusetts, USA (1986).
Haltiner et al., "A Novel Strategy for Constructing Clustered Point Mutations," 13(3) Nucleic Acids Research 1015, 1985.
Chen and Seeburg, "Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA," 4(2) DNA 165, 1985.
Lim and Pene, "Optimal Conditions for Supercoil DNA Sequencing with the Escherichia coli DNA Polymerase I Large Fragment," 5 Gene Anal. Techniques 32, 1988.
Hsiao, "A Fast and Simple Procedure for Sequencing Double-Stranded DNA with Sequenase," 19(10) Nucleic Acids Research 2787, 1991.
Hattori and Sakaki, "Dideoxy Sequencing Method Using Denatured Plasmid Template", 152 Anal. Biochem. 232-238 1986.

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