DNA cycle sequencing

Details DNA cycle sequencing DNA cycle sequencing

1995-05-18

1998-04-21

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435 5, 424 941, 536 243, 536 2433, 536 2432, C12Q 168, C12Q 170, C12P 1934, A61K 3854

Patent

active

057416400

ABSTRACT:
Method for sequencing DNA which includes the following steps: providing a polynucleotide primer complementary to a region of the DNA to be sequenced providing the DNA to be sequenced, and contacting that primer and DNA together in the presence of a DNA polymerase and between 1 and 3 dNTPs, at least one of the dNTPs being labeled. The primer and DNA are contacted under conditions which allow extension of the primer by addition of one or more of the dNTPs to the primer to form an extended primer. The primer and DNA are then dissociated, generally by heating, and the contacting and dissociating steps repeated a plurality of times (usually 10-200 times). Finally, the extended primer is contacted with the DNA in the presence of a DNA polymerase (which is generally the same polymerase as used in the initial labeling step) all four dNTPs and a chain terminating agent.

REFERENCES:
patent: 4795699 (1989-01-01), Tabor et al.
patent: 4851331 (1989-07-01), Vary et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5075216 (1991-12-01), Innis et al.
Borowi-Borowski et al. Biochemistry 29:2992-2999, 1990.
Kim et al. Korean Biochem. J. 26 (6) 579-82, Abstract cited (of interest), 1993.
Donovan et al., "Detection of the product of the polymerase chain reaction using alkaline phosphatase," J. Cell. Biochem. Suppl 13E, 282 (1989) (Abstact).
Higuichi and Ochman, Nucleic Acids Research 17:5865 (1989).
Innis et al., Proc. Natl. Acad. Sci. USA 85:9436-9440 (1988).
Khrishnan et al., Nucleic Acids Research 19:1153.
Maniatis et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York, New York, pp. 134-135 (1982).
Mead et al., Abstract No. 99, Human Genome II, Oct. 22, 1990.
Murray, Nucleic Acids Research 17:889 (1989).
Smith et al., BioTechniques 9:52 (1990).
Spurgeon and Burke, Abstract 125, Human Genome II, Oct. 22, 1990.
Straus and Zagursky, BioTechniques 10:376 (1991).

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