DNA cycle sequencing

Details DNA cycle sequencing DNA cycle sequencing

1994-04-14

1998-05-26

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435196, 935 77, 935 78, C12Q 168, C12P 1934, C12N 916

Patent

active

057562857

ABSTRACT:
Method for determining the nucleotide base sequence of a DNA molecule by amplifying that DNA, contacting the products of the amplifying reaction with an alkaline phosphatase, and determining the nucleotide base sequence of any polynucleic acid products of the amplifying reaction.

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Innis et al., PNAS(USA) 85:9436-9440(Dec. 1988).
Donovan et al., "Detection of the Product of the Polymerose chain reaction using Alkaline Phosphatase . . . ", J. Cell Biochem. Suppl 13E, 282 (1989) (Abstr).
Higuchi et al., "Production of Single-Stranded DNA Templates . . . " Nuc Acids Res. 17 (14):5865 (1989).
Maniatis et al., Molecular Cloning: 2 Lab. Manual, Cold Spring Harbor NY, 1982, pp. 134-135.

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