Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1990-04-09
1993-06-08
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435 691, 4353201, 536 2374, 935 47, 935 48, C12N 964, C12N 1581, C12P 2100
Patent
active
052178919
ABSTRACT:
DNA constructs that are useful for providing secretory expression of heterologous polypeptides in yeast comprising a DNA sequence that includes a Kluyveromyces .alpha.-factor leader sequence linked to the heterologous polypeptide by a yeast processing signal. Constructs employing the K. lactis .alpha.-factor leader and processing signal sequences, with and without spacer, linked to prochymosin are exemplified.
REFERENCES:
Zsebo, K. M., et al., Protein Secretion from Saccharomyces Cerevisiae Directed by the Prepo-.alpha.-factor leader Region, (1986) J. Biol. Chem. 261:5858-5865.
Brake, A. J. et al., .alpha.-Factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae, (1984) PNAS 81:4642-4646.
Baldari, C., et al., A novel leader peptide which allows efficient secretion of a fragment of human interleukin 1.beta. in Saccharomyces cerevisiae, (1987) EMBO 6:229-234.
Vlasuk, G. P., et al., Expression and Secretion of Biologically Active Human Atrial Natriuretic Peptide in Saccharomyces cerevisiae, (1986) J. Biol. Chem. 261:4789-4796.
Hitzeman, R. A., et al., Construction of Expression Vectors for Secretion of Human Interferons by Yeast (1986) Methods Enzymol. 119:424-431.
Guarente, L. & Ptashne, M. Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae, (1981) Proc. Nat'l Acad. Sci. USA 75:2199-2203.
Tokunaga, M., et al., A Novel Yeast Secretion Vector Utilizing Secretion Signal of Killer Toxin Encoded on the Yeast Linear DNA Plasmid PGKL1 (1987) Biochem. & Biophy. Res. Comm. 144:613-619.
Verbakel, J. M., et al., Construction of expression of Saccharomyces cerevisiae: application for synthesis of poliovirus protein VP2, (1987) Gene 61:207-215.
Ernst, J. F., Improved Secretion of Heterologous Proteins by Saccharomyces cerevisiae: Effects of Promoter Substitution in Alpha-Factor Fusions, (1986) DNA, 5:483-491.
Stark, M. J. R., et al., Neucleotide Sequence and transcription analysis of a linear DNA plasmid associated with the killer character of the yeast Kluyveromyces lactis, (1984) Nucleic Acids Research 15:6011-6030.
Brake Anthony J.
Van den Berg Johan A.
Chiron Corporation
Gist-Brocades
LeGuyader John L.
Rae-Venter Barbara
Schwartz Richard A.
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