DNA constructs and methods of producing xylanolytic enzymes

Details DNA constructs and methods of producing xylanolytic enzymes DNA constructs and methods of producing xylanolytic enzymes

1996-08-16

1998-06-23

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435200, 4352523, 43525231, 4353201, 536 232, C12N 924, C12N 120, C12N 1500, C07H 2104

Patent

active

057704244

ABSTRACT:
The present invention is directed to isolated nucleic acid constructs comprising a nucleic acid sequence encoding xylanolytic enzymes derived from a strain of Bacillus agaradherens, recombinant vectors and host cells comprising such constructs, and methods for obtaining xylanolytic enzymes.

REFERENCES:
Yu et al. (1990) Nucleotide sequence and analysis of a xylanase gene (xynS) from alkali-tolerant Bacillus sp. YA-14 and comparison with other xylanases. Genbank submission Acc No.: X59059, Dec. 4, 1990.
Na et al. (1990) Recombinant plasmid DNA containing xylanase and betat-xylosidase gene of Bacillus sp. YA-14, Sanop Misaengmul Hakhoechi 18(2): 195-198, Jan. 1990.
Fukusaki et al. (1984) The complete nucleotide sequence of the xylanase gene (xynA) of Bacillus pumilus, FEBS Letters 171(2): 197-201, Jun. 1984.
Zappe et al. (1990) Nucleotide sequence of a Clostridium acetobutylicum P262 xylanase gene (xynB), Nucleic Acids Research 18(8): 2179, Jun. 1990.
Ko et al. (1992) Site-directed mutagenesis at aspartate and glutamate residues of xylanase from Bacillus pumilus, Biochem. J. 288: 117-121, Nov. 15, 1992.
Sakka et al. (1993) Nucleotide Sequence of the Clostridium stercorarium xynA Gene Encoding Xylanase A: Identification of Catalytic and Cellulose Binding Domains, Biosci. Biotech. Biochem. 57 (2): 273-277. Feb. 1993.
Rudinger, J. (1976) Characteristics of the amino acids as components of a peptide hormone sequence, In Peptide Hormones, Ed. J. A. Parsons, pp. 1-7, Jun. 1976.
Mierendorf et al. (1987) Gene Isolation by Screening lambda gt 11 Libraries with Antibodies, In Guide to Molecular Cloning Techniques, Eds. S. L. Berger and A. R. Kimmel. pp. 458-469, Jan. 1987.

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