Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-09-29
1999-11-30
Ketter, James
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 8, 435370, 536 241, C12Q 168
Patent
active
059940611
ABSTRACT:
Methods for screening for a drug that increases expression of the human apolioprotein (apo) AI gene and related DNA constructs. A first method has the steps of: (a) introducing into mammalian cells a DNA construct including, functionally joined together in the 5'.fwdarw.3' direction of transcription, (i) at least one copy of a drug-responsive element (DRE) having at least about 80% homology with the DNA sequence 5'-G/C N T/G A/G GCTGGG-3', (ii) a heterologous promoter, (iii) a reporter gene and (iv) an untranslated region including a functional polyadenylation signal; (b) growing a first culture of the cells in the absence of drug; (c) lysing the first culture to produce a first extract; (d) assaying the first extract for activity of a protein encoded by the reporter gene; (e) growing a second culture of the cells in the presence of the drug; (f) lysing the second culture to produce a second extract; (g) assaying the second extract for activity of the protein encoded by the reporter gene; and (h) comparing the activities of the first extract and the second extract. A second method uses a different DNA construct including (i) a promoter region of the human apo AI gene including at least one copy of a drug-responsive element (DRE) having at least about 80% homology with the DNA sequence 5'-G/C N T/G A/G GCTGGG-3', (ii) a reporter gene and (iii) an untranslated region including a functional polyadenylation signal. A third method involves stably maintaining the introduced DNA in the cells.
REFERENCES:
patent: 4393133 (1983-07-01), Knowles et al.
patent: 4677057 (1987-06-01), Curtiss et al.
patent: 4801531 (1989-01-01), Frossard
patent: 4828986 (1989-05-01), Smith et al.
patent: 4943527 (1990-07-01), Protter et al.
patent: 5059528 (1991-10-01), Bollen et al.
patent: 5220006 (1993-06-01), Ross et al.
patent: 5320968 (1994-06-01), Seman
patent: 5378822 (1995-01-01), Bradfield et al.
patent: 5408038 (1995-04-01), Smith et al.
patent: 5580722 (1996-12-01), Foulkes et al.
Sastry et al. 1988 Mol. Cell. Biol. 8(2): 605-614.
Fujii-Kuriyama et al. 1986 Proc. Nat'l. Acad. Sci USA 83: 8044-8048.
Chen, et al., Circulation 90, I-570, 3069 (Oct. 1994).
Karathanasis et al., Nature (London) 304: 371-373 (1983).
Breslow et al., Proc. Nat. Acad. Sci. USA 79: 6861-6865 (1982).
Sogawa et al., Proc. Natl. Acad. Sci. USA 83: 8044-8048 (1986).
Fujisawa-Sehara et al., Proc. Natl. Acad. Sci. USA 85: 5859-5863 (1988).
Sigurdsson et al., Arteriosclerosis and Thrombosis 12: 1017-1022 (1992).
Tuteja et al., FEBS Letters 304: 98-101 (1992).
Jeenah et al., Mol. Biol. Med. 7: 233-241 (1990).
Pagani et al., J. Lipid Res. 31: 1371-1377 (1990).
Sastry et al., Mol. Cell. Biol. 8: 605-614 (1988).
Widom et al., Mol Cell. Biol. 11: 677-687 (1991).
Smith et al., J. Clin. Invest. 89: 1796-1800 (1992).
Papazafiri et al., J. Biol. Chem. 266: 5790-5797 (1991).
Angotti et al., J. Biol. Chem. 269: 17,371-17,374 (1994).
Tam, Atherosclerosis 91: 51-61 (1991).
Tam et al., J. Biol. Chem. 260: 1670-1675 (1985).
Tam, Alcohol. Clin. Exp. Res. 16: 1021-1028 (1992).
Tam et al., Atherosclerosis 105: 235-243 (1994).
Frick et al., N. Engl. J. Med. 317: 1237-1245 (1987).
Manninen et al., Circulation 85: 37-45 (1992).
Brown, Am. J. Catdiol. 66: 11A-15A (1990).
Gordon et al., Am. J. Med. 62: 707-714 (1977).
Stampfer et al., N. Engl. J. Med. 325: 373-381 (1991).
Kussi et al., Arteriosclerosis 7: 421-425 (1987).
Moll et al., Am. J. Human Genet. 44: 124-139 (1989).
Hamsten et al., Atherosclerosis 60: 199-208 (1986).
Gotto et al., Methods Enzymol. 128: 3-41 (1986).
Miller et al., Nature (London) 314: 109-111 (1985).
Glomset. Adv. Intern. Med. 25:91-116 (1980).
Rubin et al., Nature (London) 353: 265-267 (1991).
Knuiman et al., Arteriosclerosis 7: 612-619 (1987).
Berg et al., Clin. Chim. Acta 161: 165-171 (1986).
Davidson et al., J. Lipid Res. 29: 1511-1522 (1988).
Haddad et al., J. Biol. Chem. 26: 13,268-13,277 (1986).
Staels et al., J. Lipid Res. 30: 1137-1145 (1989).
Luoma et al., Acta Med. Scand. 214: 103-109 (1983).
Luoma, Pharm & Toxic. 65: 243-249 (1988).
GeneLight.TM. Plasmids Technical Manual, Promega Corporation, WI pp. 1-39 (1991).
Gorman et al., Mol. Cell Biol. 2: 1044-1051 (1982).
Scatchard, Ann. N.Y. Acad. Sci. 51: 660-672 (1949).
Wilson et al., Plasmid 33: 198-207 (1995).
Colbere-Garapin et al., J. Mol. Biol. 150: 1-14 (1981).
Jimenz et al., Nature (London) 287: 869-871 (1980).
de Wet et al., DNA 3, 437-447 (1984).
PCR Protocols: A Guide to Methods and Applications, Innis et al. (ed.), Academic Press, San Diego, CA pp. 13-20 (1990).
Sambrook et al.,. Molecular Cloning: A Laboratory Manual (2nd edition), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY pp. 14.1-14.21 (1989).
Bartalena, Mol. Endocrinol. 6: 935-942 (1992).
McKnight et al., J. Biol. Chem. 254: 9050-9058 (1979).
Dignam et al., Nucl. Acids Res. 11: 1475-1489 (1983).
Lowry et al., J. Biol. Chem. 193: 265-275 (1951).
Faisst et al., Nucl. Acids Res. 20: 3-26 (1992).
Whitlock, Jr., Annu. Rev. Pharmacol. Toxicol. 30: 251-277 (1990).
Singh et al., Biotechniques 7:252-261 (1989).
Tam Shui-Pang
Zhang Xia
Ketter James
Queen's University at Kingston
Steeg Carol Miernicki
Yucel Irem
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