DNA construct encoding CE7-specific chimeric T cell receptor

Details DNA construct encoding CE7-specific chimeric T cell receptor DNA construct encoding CE7-specific chimeric T cell receptor

2008-02-08

2008-11-04

Blanchard, David J. (Department: 1643)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S023530, C424S134100, C424S135100, C424S143100, C424S155100, C435S069600, C435S069700, C435S235100, C435S320100, C435S328000, C435S344100, C435S372000, C435S372300, C530S387300, C530S388220, C530S388850

Reexamination Certificate

active

07446191

ABSTRACT:
Genetically engineered, CE7-specific redirected immune cells expressing a cell surface protein having an extracellular domain comprising a receptor which is specific for CE7, an intracellular signaling domain, and a transmembrane domain, and methods of use for such cells for cellular immunotherapy of CE7+neuroblastoma are disclosed. In one embodiment, the immune cell is a T cell and the cell surface protein is a single chain FvFc:receptor where Fv designates the VHand VLchains of a single chain monoclonal antibody to CE7 linked by peptide, Fc represents a hinge-CH2-CH3 region of a human IgG1, andrepresents the intracellular signaling domain of the zeta chain of human CD3. DNA constructs encoding a chimeric T-cell receptor and a method of making a redirected T cell expressing a chimeric T cell receptor by electroporation using naked DNA encoding the receptor are also disclosed.

REFERENCES:
patent: 5912172 (1999-06-01), Eshhar et al.
patent: 6410319 (2002-06-01), Raubitschek et al.
patent: 9715669 (1997-05-01), None
patent: 00/23573 (2000-04-01), None
patent: 00/31239 (2000-06-01), None
Nolan et al., Clinical Cancer Research, 5(12):3928-3941, 1999.
Hombach et al., Int. J. Mol. Med., 2(1):99-103, 1998.
Jensen, M. et al., “CD20 is A Molecular Target For scFvFc: Receptor Redirected T Cells: Implications For Cellular Immunotherapy of CD2+ Malignancy,” Biology of Blood and Marrow Transplantation, 4:75-83, 1998.
Abken, H. et al., “Chimeric T-Cell Receptors: Highly Specific Tools To Target Cytotoxic T-Lymphocytes To Tumour Cells,” Cancer Treatment Reviews, 23:97-112, 1997.
Nicholson, Ian C. et al., “Construction And Characteristics Of A Functional CD19 Specific Single Chain Fv Fragment . . . ,” Molecular Immunology, vol. 34, No. 16-17, 1157-1165, 1997.
Jensen, M. C. et al., Abstract ™98: “Targeting Pre-B Acute Lymphoblastic Leukemia With T Cell Clones Engineered To Express A CD19-Specific Chimeric Immunoreceptor,” Blood, 96(11), p. 264, Nov. 16, 2000.
Amstutz, H. et al., “Production And Characterization Of A Mouse/Human Chimeric Antibody Directed Against Human Neuroblastoma,” Int. J. Cancer, 53:147-152, 1993.
D'Uscio et al., Journal of Immunological Methods, 146:63-70, 1992.
Heuser et al., Gene Therapy, 10:1408-1419, 2003.
Cross et al., Molecular Biology of the Cell, 13:2881-2893, 2002.

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