Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2006-01-10
2006-01-10
Crouch, Deborah (Department: 1632)
Chemistry: molecular biology and microbiology
Vector, per se
C536S023720, C536S024100
Reexamination Certificate
active
06984518
ABSTRACT:
A transgenic, non-human mammal useful for assessing the effect of candidate chemotherapeutic drugs on the growth of brain tumors in vivo is provided. Incorporated into the genome of the transgenic mammal, which preferably is a rodent, is a transgene which comprises a promoter comprising the nuclear factor binding region of the RR2cis acting element of a fibroblast growth factor1B (FGF1B) promoter. Operably linked to the promoter is reporter gene comprising a sequence which encodes the SV40large T antigen. A transgenic, non-human mammal useful for identifying and isolating FGF1producing brain cells. Incorporated into the genome of these transgenic animals is a transgene which comprises a promoter comprising the nuclear factor binding region of the RR2cis acting element of an fibroblast growth factor1B (FGF1B) promoter. Operably linked to the promoter is reporter gene comprising a sequence which encodes a protein or polypeptide other than an SV40large T antigen. A method of obtaining neural stem cells from a sample of cells obtained from an animal is also provided. Such method comprises introducing the FGF1B-detector transgene into a sample of cells that have been obtained from the animal, and assaying for expression of the detectable marker in the cells, wherein cells that express the marker are neural stem cells. The cells which express the detectable marker can then be isolated from the population to provide a sub-population of neural stem cells.
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Calfee Halter & Griswold LLP
Crouch Deborah
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