Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1996-05-31
1998-10-27
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
4352523, 4353201, 435325, 536 232, 935 22, C12N 910, C12N 120, C12N 500, C07H 2104
Patent
active
058277137
ABSTRACT:
Chondrotin 6-sulfotransferase (C6ST), for transferring sulfate group to the hydroxyl group at C-6 position of N-acetylgalactosamine residue or galactose residue of glycosaminoglycan, was purified from a culture liquid of chick embryo chondrocytes to determine its partial amino acid sequences. Partial cDNA of C6ST was amplified from poly(A).sub.+ prepared from the chondrocytes, by means of PCR by using oligonucleotide primers prepared on the basis of the determined sequences. Entire length cDNA of C6ST was obtained from a cDNA library by means of hybridization by using an obtained cDNA fragment as a probe.
REFERENCES:
"Current Protocols in Molecular Biology." Ausubel, F.M. (Ed.), Wiley & Sons, New York, USA, vol. 1, 1987, pp. 6.4.1-6.4.10.
Rudinger, J. (1976) Characteristics of the amino acids as components of a peptide hormone sequence, In Peptide Hormones, Ed. J. A. Parsons, pp. 1-7, Jun. 1976.
Fukuta et al. 1995 Molecular Cloning and Expression of Chick Chondrocyte Chondroitin 6-Sulfotransferase, J. Biol. Chem. 270 (31): 18575-18580, Aug. 4, 1995.
Sugumaran et al. (1995) Purification, Photoaffinity Labeling, and Characterization of a Single Enzyme for 6-Sulfation of both Chondroitin Sulfate and Keratan Sulfate, J. Biol. Chem. 270 (38): 22483-22487, Sep. 22, 1995.
Habuchi et al. (1993) Purification of Chondroitin 6-Sulfotransferase Secreted from Cultured Chick Embryo Chondrocytes, J. Biol. Chem. 268 (29): 21968-21974, Oct. 15, 1993.
Habuchi and Miyashita (1982) Separation and Characterization of Chondroitin 6-Sulfotransferase and Chondroitin 4-Sulfotransferase from Chick Embryo Cartilage, Biochimica et Biophysica Acta 717: 414-421, Aug. 1982.
Fukuta Masakazu
Habuchi Osami
Seikagaku Kogyo Kabushiki Kaisha
Stole Einar
Wax Robert A.
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