DNA coding for growth-inhibitory factor and use thereof

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 911, 4351723, 4352402, 4352523, 4353201, 536 231, 536 234, 536 235, 536 241, C12P 2102, C12N 1512, C12N 121, C12N 510

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054895146

ABSTRACT:
A novel protein of GIF having growth-inhibitory action, which is present in the brain of normal subjects but absent in the brain of patients with Alzheimer disease, was cloned from a human-cerebral cDNA library and human genomic DNA library. Further, Escherichia coli was transformed with a vector integrated by the cDNA to express GIF. The DNA coding for the cloned GIF can be used in the genetic diagnosis and gene therapy for Alzheimer disease, and the expression of the DNA in the transformant can bring about a large production of GIF.

REFERENCES:
patent: 4828990 (1989-05-01), Higashi et al.
patent: 5214031 (1993-05-01), Uchida et al.
Vol. 150, No. 3, (1988) Biochemical and Biophysical Research Communications, pp. 1263-1267--Alzheimer's Disease Brain Extract Stimmulates . . . From Neonatal Rats, Uchida, et al.
Brain Research 481 (1989), pp. 190-193, Neurotrophic action of Alzheimer's Disease brain . . . Cortical Neurons, Uchida, et al.

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