DNA coding for endo-β-galactosidase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C536S023200

Reexamination Certificate

active

11407596

ABSTRACT:
A full-length cDNA is obtained by analyzing the partial amino acid sequence of purified endo-β-galactosidase C, constructing primers based thereon, effecting PCR with the use of the genomic DNA ofClostridium perfringensas a template to thereby obtain a fragment of cDNA encoding endo-β-galactosidase, effecting cassette PCR by using the cDNA fragment thus obtained to thereby obtain the 5′-terminal and 3′-terminal domains, and further effecting PCR with the use of primers constructed on the basis of the data acquired above.

REFERENCES:
patent: 5925541 (1999-07-01), Goldstein
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patent: WO 98/11246 (1998-03-01), None
Sequence search alignment between Acc. No. Q59328 and SEQ ID No. 2.
Ogawa, et al.Cell Technology, Special Issue: New Stage of Organ Transplantation; From Xenotransplantation to Cell Transplantation, vol. 19, No. 6, Jun. 2000, pp. 344, 846-848. English translation of title, abstract, selected sections and figure legend.
Ogawa, et al. “Endo-beta-galactosidase C precursor,” Database accession No. Q9KWF3, XP002238200, Oct. 1, 2000.
Supplementary European Search Report issued in a related foreign application dated Apr. 14, 2003.
Ogawa, H., et al. “Molecular Cloning of Endo-β-galactosidase C and Its Application in Removing α-Galactosyl Xenoantigen from Blood Vessels in the Pig Kidney,”The Journal of Biological Chemistry, vol. 275, No. 25, Jun. 2000. pp. 19368-19374.
The 5thCongress of the International Xenotransplantation Association. “Transplantation Proceedings,” Oct. 24-28, 1999, Nagoya, Japan.

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