DNA coding for endo-β-galactosidase

Details DNA coding for endo-β-galactosidase DNA coding for endo-β-galactosidase

2008-07-01

2008-07-01

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C536S023200

Reexamination Certificate

active

07393672

ABSTRACT:
A full-length cDNA is obtained by analyzing the partial amino acid sequence of purified endo-β-galactosidase C, constructing primers based thereon, effecting PCR with the use of the genomic DNA ofClostridium perfringensas a template to thereby obtain a fragment of cDNA encoding endo-β-galactosidase, effecting cassette PCR by using the cDNA fragment thus obtained to thereby obtain the 5′-terminal and 3′-terminal domains, and further effecting PCR with the use of primers constructed on the basis of the data acquired above.

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Sequence search alignment between Acc. No. Q59328 and SEQ ID No. 2.
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Ogawa, et al. “Endo-beta-galactosidase C precursor,” Database accession No. Q9KWF3, XP002238200, Oct. 1, 2000.
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Ogawa, H., et al. “Molecular Cloning of Endo-β-galactosidase C and Its Application in Removing α-Galactosyl Xenoantigen from Blood Vessels in the Pig Kidney,”The Journal of Biological Chemistry, vol. 275, No. 25, Jun. 2000. pp. 19368-19374.
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